Pnpla5-knockout rats exhibit reduced expression levels of proteins involved in steroid metabolism and wound healing compared to wild-type rats

文献类型： 外文期刊

第一作者： Liu, Zhi-Guo

作者： Liu, Zhi-Guo;Hu, Yan-Qing;Li, Kui;Mu, Yu-Lian;Wu, Tian-Wen;Li, Kui

作者机构：

关键词： Pnpla5; Pnpla5-knockout rats; Apoptosis; iTRAQ; Wound healing; Steroid metabolism; Membrane attack complex

期刊名称：BMC GENOMICS （ 影响因子：4.547； 五年影响因子：4.931 ）

ISSN： 1471-2164

年卷期： 2022 年 23 卷 1 期

页码：

收录情况： SCI

摘要： Background Patatin-like phospholipase domain containing 5 (PNPLA5) is a newly-discovered lipase. Although the PNPLA family plays critical roles in diverse biological processes, the biological functions of PNPLA5 mostly unknown. We previously found that the deletion of Pnpla5 in rats causes a variety of phenotypic abnormalities. In this study, we further explored the effects of Pnpla5 knockout (KO) on male rats. Results The body weight and testicular or epididymal tissue weight of three to six 3-month-old Pnpla5 KO or wild-type (WT) male Sprague-Dawley rats were measured. The protein expression levels were also measured via western blotting and iTRAQ (isobaric tags for relative and absolute quantitation) analyses. No significant difference between Pnpla5 KO and WT rats, regarding body weight, testicular or epididymal tissue weight, or hormone levels, were found. However, the relative testicular tissue weight of the KO (Pnpla5(-/-)) rats was higher (P < 0.05) than that of WT rats. Significant increases in apoptotic cells numbers (P < 0.001) and BAX and Caspase-9 expression levels were observed in the testicular tissue of Pnpla5(-/-) rats. Moreover, iTRAQ analysis revealed that the levels of proteins involved in steroid metabolism and wound healing were significantly decreased in Pnpla5(-/-) rats. Conclusion This study revealed that Pnpla5 knockout induced apoptosis in rat testes. We also ascertained that Pnpla5 plays an important role in lipid metabolism, wound healing, and affects reproductive organs negatively, providing new target genes and pathways that can be analyzed to unravel the biological function of Pnpla5.

分类号：

  • 相关文献
作者其他论文 更多>>
  • EXPERT expands prime editing efficiency and range of large fragment edits

    作者：Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Xiong, Youcai;Su, Yinyu;He, Ruigao;Han, Xiaosong;Li, Sheng;Liu, Minghuan;Xi, Xiaoning;Liu, Zijia;Wang, Heng;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Ruan, Jinxue;Han, Xiaosong;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Xie, Shengsong;Xu, Xuewen;Li, Xinyun;Zhao, Shuhong;Li, Kui;Zhang, Jifeng;Xu, Jie

    关键词：

  • Genome-Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells

    作者：Zhu, Xiusheng;Huang, Lei;Li, Guoli;Deng, Biao;Wang, Xiaoxiao;Yang, Hu;Zhang, Yuanyuan;Wen, Qiuhan;Wang, Chao;Zhang, Jingshu;Li, Kui;Liu, Yuwen;Deng, Biao;Deng, Biao;Zhao, Yunxiang

    关键词：cis-regulatory elements; high-throughput; iPSCs; silencer; Ss-STARR-seq

  • Transcriptome analysis reveals no obvious unintended effects in the spleen of CRISPR/Cas9-mediated CD163 and pAPN double-knockout pigs

    作者：Liu, Zhi-Guo;Wang, Nan;Wang, Wan-Jie;Chi, Shun-Shun;Nie, Yu-Xin;Yuan, Mao-Sha;Sun, Ya-Ru;Mu, Yu-Lian;Li, Kui;Wang, Nan;Huang, Lei;Chi, Shun-Shun;Li, Kui

    关键词：CD163; pAPN; Double-knockout; Gene editing; Pig; Spleen; Unintended effects; Transcriptomics

  • Integrating large-scale meta-GWAS and PigGTEx resources to decipher the genetic basis of 232 complex traits in pigs

    作者：Xu, Zhiting;Lin, Qing;Cai, Xiaodian;Zhong, Zhanming;Teng, Jinyan;Zeng, Haonan;Gao, Yahui;Wei, Chen;Zhou, Jun;Zhang, Wenjing;Zhang, Xiaoke;Shi, Shaolei;Wu, Jun;Diao, Shuqi;Liu, Yuqiang;Pan, Xiangchun;Feng, Xueyan;Liu, Ruiqi;Su, Zhanqin;Chang, Chengjie;Zhu, Qianghui;Wu, Yuwei;Zhang, Hao;Yuan, Xiaolong;Li, Jiaqi;Zhang, Zhe;Li, Bingjie;Gao, Yahui;Liu, George E.;Gao, Yahui;Ma, Li;Cai, Zexi;Bai, Zhonghao;Su, Guosheng;Sahana, Goutam;Lund, Mogens Sando;Fang, Lingzhao;Wang, Xiaoqing;Shi, Liangyu;Zhao, Fuping;Wang, Xue;Wang, Yi;Zhang, Zipeng;Ding, Xiangdong;Lin, Yu;Tang, Guoqing;Li, Mingzhou;Li, Xuewei;Liu, Shuli;Yin, Hongwei;Bai, Lijing;Li, Kui;Zhou, Zhongyin;Wang, Qishan;Pan, Yuchun;Xu, Zhong;Peng, Xianwen;Mei, Shuqi;Mo, Delin;Liu, Xiaohong;Chen, Yaosheng;Liu, Yang;Xiang, Ruidong;Xiang, Ruidong;Shen, Xia;Shen, Xia;Shen, Xia;Li, Pinghua;Huang, Ruihua;Li, Pinghua;Huang, Ruihua;Ballester, Maria;Crespo-Piazuelo, Daniel;Amills, Marcel;Clop, Alex;Amills, Marcel;Karlskov-Mortensen, Peter;Fredholm, Merete;Zhang, Qin;Zhao, Yunxiang

    关键词：pig; meta-GWAS; PigGTEx; molQTL; complex traits

  • Multi-dimensional annotation of porcine variants using genomic and epigenomic features in pigs

    作者：Yin, Hongwei;Yang, Liu;Zhao, Qianyi;Yao, Wenye;Bai, Lijing;Li, Kui;Fang, Lingzhao;Yao, Wenye;Groenen, Martien A. M.;Madsen, Ole;Teng, Jinyan;Gao, Yahui;Xu, Zhiting;Lin, Qing;Diao, Shuqi;Li, Jiaqi;Zhang, Zhe;Zhou, Huaijun;Guan, Dailu;Liu, Xiaohong;Zhao, Fuping;Zhou, Zhongyin;Wang, Qishan

    关键词：Genomic variants; Gene expression and splicing; Complex traits; Non-coding regions; Epigenome

  • DPImpute: A Genotype Imputation Framework for Ultra-Low Coverage Whole-Genome Sequencing and its Application in Genomic Selection

    作者：Zheng, Weigang;Wang, Chao;Dong, Wenjun;Liu, Yuwen;Zheng, Weigang;Wang, Chao;Dong, Wenjun;Liu, Yuwen;Zheng, Weigang;Ma, Wenlong;Chen, Zhilong;Wang, Chao;Sun, Tao;Dong, Wenjun;Zhang, Song;Tang, Zhonglin;Li, Kui;Liu, Yuwen;Zheng, Weigang;Ma, Wenlong;Chen, Zhilong;Wang, Chao;Sun, Tao;Dong, Wenjun;Zhang, Song;Tang, Zhonglin;Li, Kui;Liu, Yuwen;Zhang, Wenjing;Tang, Zhonglin;Liu, Yuwen;Zhao, Yunxiang

    关键词：breeding models; genetic screening of embryos; genomic selection; genotype imputation; single cell genotype

  • Innovation in Agricultural Water Pricing Systems in China Based on Irrigation Benefits

    作者：Feng, Xin;Li, Kui;Feng, Xin;Liu, Zixuan;Jiang, Wenlai;Liu, Yang

    关键词：irrigation benefit sharing coefficient; grain crops; C-D production function; sharing of agricultural water prices; sustainable agricultural development

微信小程序