Regulation of Dietary Protein Solubility Improves Ruminal Nitrogen Metabolism In Vitro: Role of Bacteria-Protozoa Interactions

文献类型： 外文期刊

第一作者： Zhang, Zhenbin

作者： Zhang, Zhenbin;Wei, Wenjun;Yang, Sihan;Huang, Zeliang;Li, Chuang;Yu, Xiang;Qi, Ruxin;Wang, Mengzhi;Zhang, Zhenbin;Wang, Mengzhi;Zhang, Xin;Liu, Wujun;Loor, Juan J.

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关键词： dietary interventions; soluble protein; nitrogen metabolism; rumen bacteria; ciliate protozoa

期刊名称：NUTRIENTS （ 影响因子：6.706； 五年影响因子：7.185 ）

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年卷期： 2022 年 14 卷 14 期

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收录情况： SCI

摘要： Precision dietary interventions (e.g., altering proportions of dietary protein fractions) has significant implications for the efficiency of nutrient use in ruminants, as well as lowering their environmental footprint, specifically nitrogen (N) emissions. Soluble protein (SP) is defined as the protein fraction that is rapidly degraded in the rumen (e.g., non-protein N and true protein), and our previous study found that regulating SP levels could improve N efficiency in Hu sheep. Thus, the present study was conducted to explore in vitro how protein fractions with different SP levels modulate the rumen microbial community and its association with N metabolism. Four dietary treatments with different SP proportions and similar crude protein (CP) content (similar to 14%) were formulated (% of CP): 20 (S20), 30 (S30), 40 (S40) and 50 (S50). Results showed that NH3-N content increased with increasing SP levels at 4, 12 and 24 h; TVFA, acetate, propionate and valerate were higher in S30 and S40 (p < 0.05) and had quadratic effects (p < 0.05). Moreover, dry matter digestibility (DMD) and N digestibility (ND) were all decreased with S20 and S50 (p < 0.05). The S30 and S40 treatments increased the abundance of Bacteroidetes and Prevotella (Prevotella_ruminicola) but decreased the abundance of Firmicutes and Proteobacteria (p < 0.05). Bacterial pathways related to amino acid and fatty acid metabolism also were enriched with S30 and S40. The abundance of Entodinium was increased with S30 and S40 and had a positive correlation with Prevotella, and these two genera also played an important role in N metabolism and VFA synthesis of this study. In conclusion, bacterial and protozoal communities were altered by the level of SP (% of CP), with higher SP levels (similar to 50% of CP) increasing the microbial diversity but being detrimental to rumen N metabolism.

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