Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine Renibacterium sp. Suitable for Chitooligosaccharides Preparation

文献类型： 外文期刊

第一作者： Zhang, Lin-Lin

作者： Zhang, Lin-Lin;Xiao, Xin-Feng;Zhang, Wen-Xiu;Shi, Yi-Qian;Jiang, Xiao-Hua;Zhou, Hai-Xiang;Wang, Zhi-Peng

作者机构：

关键词： chitosanase; cold adaptation; chitooligosaccharide;

Renibacterium sp.& nbsp;

期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）

ISSN：

年卷期： 2021 年 19 卷 11 期

页码：

收录情况： SCI

摘要： (1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones with high stability are still rather rare but required. (2) Methods: A novel chitosanase named CsnY from marine bacterium Renibacterium sp. Y82 was expressed in Escherichia coli, following sequence analysis. Then, the characterizations of recombinant CsnY purified through Ni-NTA affinity chromatography were conducted, including effects of pH and temperature, effects of metal ions and chemicals, and final product analysis. (3) Results: The GH46 family chitosanase CsnY possessed promising thermostability at broad temperature range (0-50 & DEG;C), and with optimal activity at 40 & DEG;C and pH 6.0, especially showing relatively high activity (over 80% of its maximum activity) at low temperatures (20-30 & DEG;C), which demonstrated the cold-adapted property. Common metal ions or chemicals had no obvious effect on CsnY except Mn2+ and Co2+. Finally, CsnY was determined to be an endo-type chitosanase generating chitodisaccharides and -trisaccharides as main products, whose total concentration reached 56.74 mM within 2 h against 2% (w/v) initial chitosan substrate. (4) Conclusions: The results suggest the cold-adapted CsnY with favorable stability has desirable potential for the industrial production of COS.

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