An insertion in intron 3 of nAChR alpha 6 subunit is associated with spinosad resistance in the western flower thrips Frankliniella occidentalis

文献类型： 外文期刊

第一作者： Zhang, Kun

作者： Zhang, Kun;Yuan, Jiangjiang;Wan, Yanran;Wang, Jing;Zheng, Xiaobin;Zhang, Youjun;Wu, Qingjun;Zhang, Kun;Wu, Shaoying;Zhang, Kun;Wu, Shaoying;Liang, Pei;Zhou, Xuguo

作者机构：

关键词： inverted repeat; nAChRs; truncated?6 subunit; spinosyn; insecticide resistance; pesticide

期刊名称：ENTOMOLOGIA GENERALIS （ 影响因子：6.9； 五年影响因子：5.2 ）

ISSN： 0171-8177

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Derived from natural products of a soil bacteria, spinosad is a highly effective insecticide with a broad spectrum and a sound environmental profile. The mode of action of spinosad is the disruption of a neural mechanism, targeting primarily at the alpha 6 subunit of nicotinic acetylcholine receptors (nAChRs) of the insect nervous system. Insect pests, including the western flower thrips, Frankliniella occidentalis (Pergande), however, have developed resistance to spinosad by producing truncated alpha 6 subunits. Here, we cloned and compared the splicing variants and genomic DNAs of alpha 6 subunit using a pair of spinosad susceptible (Ivf03) and resistant (NIL-R) near-isogenic F. occidentalis lines with the same genetic background. The resultant structural differences in alpha 6 subunit between Ivf03 and NIL-R were further validated in Sf9 cells using a mini-gene construct. Results showed 100% truncation of the alpha 6 subunit transcripts in the NIL-R strain, and the presence of seven INDELs (insertions and deletions) within alpha 6 subunit between the two strains, where a 230 bp insertion in intron 3 of NIL-R had inverted repeat at both ends. In Sf9 cells, this insertion sequence was able to mediate 92.7% truncation of the mini-gene transcript. More importantly, the ratio of insertion is positively correlated with spinosad resistance levels in both lab (4) and field (8) F. occidentalis populations (y=53.28x-4.054, R2=0.9527, N=12). This study not only advances our mechanistic understanding of spinosad resistance in F. occidentalis, but also provides a diagnostic tool for monitoring the development of resistance in the field.

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