16S rRNA gene sequencing analysis reveals an imbalance in the intestinal flora of Eriocheir sinensis with hepatopancreatic necrosis disease

文献类型： 外文期刊

第一作者： Zhan, Ming

作者： Zhan, Ming;Xi, Changjun;Gong, Jie;Zhu, Mengru;Xu, Gangchun;Shen, Huaishun;Zhan, Ming;Xi, Changjun;Gong, Jie;Zhu, Mengru;Shui, Yan;Xu, Zenghong;Xu, Gangchun;Shen, Huaishun

作者机构：

关键词： Eriocheir sinensis; Hepatopancreas necrosis disease (HPND); Intestinal flora; High-throughput sequencing

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：3.306； 五年影响因子：3.251 ）

ISSN： 1744-117X

年卷期： 2022 年 42 卷

页码：

收录情况： SCI

摘要： Hepatopancreas necrosis disease (HPND) is a highly fatal disease that first appeared in Jiangsu Province, China, in 2015, and later spread to many other provinces, which had a severe impact on the culture of Chinese mitten crab (Eriocheir sinensis). Here, changes in the intestinal flora of healthy and HPND-affected Chinese mitten crabs were compared via 16S rRNA sequencing. Our findings indicated that Firmicutes, Bacteroidota, and Proteobacteria were the three dominant phyla in both healthy and HPND-affected crabs and exhibited no significant differences in alpha-diversity (richness p = 0.0892; evenness and diversity p = 0.0630). Furthermore, there were no significant changes in the abundance of Proteobacteria between the experimental groups. However, the abundance of Bacteroidota in the HPND group was significantly higher than that of the control group (HPND: 30.12%, Control: 16.60%), whereas the abundance of Firmicutes was significantly lower (HPND: 29.90%, Control: 50.55%). At the genus level, the abundance of Candidatus Bacilloplasma, Desulfovibrio, Bacteroides, and Aeromonas also differed significantly between groups (P < 0.05). Collectively, our study confirms an imbalance in the gut microbiota of Chinese mitten crabs with HPND and we speculate that this alteration may affect the metabolism and immune function of these organisms. Furthermore, we suspect that the structural changes in the intestinal flora of sick crabs observed in our study may be related to HPND.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序