Rapid and sensitive detection of quizalofop-p-ethyl by gold nanoparticle-based lateral flow immunoassay in agriproducts and environmental samples
文献类型: 外文期刊
第一作者: Liu, Xue
作者: Liu, Xue;Kong, Fanyu;Qiu, Jun;Fang, Song;Li, Jiao;Huang, Yue;Zhang, Zhongrong;Hua, Xiude;Lin, Qing;Xia, Pengliang
作者机构:
关键词: Quizalofop-p-ethyl; Monoclonal antibody; Enzyme-linked immunosorbent assay; Gold nanoparticle-based lateral flow; immunoassay; Pesticide residue
期刊名称:SCIENCE OF THE TOTAL ENVIRONMENT ( 影响因子:9.8; 五年影响因子:9.6 )
ISSN: 0048-9697
年卷期: 2023 年 857 卷
页码:
收录情况: SCI
摘要: Quizalofop-p-ethyl is a widely used herbicide that poses a threat to human health and environmental safety. In this study, anti-quizalofop-p-ethyl monoclonal antibodies (mAbs) were prepared and used to develop a gold nanoparticle-based lat-eral flow immunoassay (AuNP-LFIA) for the detection of quizalofop-p-ethyl in agriproducts and environmental samples. Four hybridoma cell lines were obtained, among which 5B6D10E11 secreted mAb with the highest sensitivity, with a 50 % inhibition concentration of 4.57 ng/mL in the indirect competitive enzyme-linked immunosorbent assay. After optimization, the AuNP-LFIA strip based on the mAb (5B6D10E11) showed a visual detection limit of 10 ng/mL, and the results could be directly determined by the naked eye within 8 min. The cross-reactivity of the AuNP-LFIA for analogs of quizalofop-p-ethyl was negligible except for quizalofop-p-acid. The established AuNP-LFIA was proven to be accurate and precise based on the recovery test. Furthermore, the detection results of AuNP-LFIA were consistent with those of ultra-high-performance liquid chromatography tandem mass spectrometry.
分类号:
- 相关文献
作者其他论文 更多>>
-
Plasmonic colorimetry and G-quadruplex fluorescence-based aptasensor: A dual-mode, protein-free and label-free detection for OTA
作者:Li, Ming;Qiu, Youxin;Liu, Guoxing;Xiao, Yu;Tian, Ye;Xiao, Yu;Fang, Song;Li, Ming
关键词:Ochratoxin A; Surface plasmon resonance; G -quadruplex; Gold nanoparticle; Dual -mode detection
-
Application of PLSR in correlating sensory and chemical properties of middle flue-cured tobacco leaves with honey-sweet and burnt flavour
作者:Li, Jing;Qiu, Jun;Pang, Xueli;Ma, Zhiyuan;Dai, Huawei;Li, Hu
关键词:Shandong flue -cured tobacco; Sensory quality; Flavour -related chemical components; Aroma -active compounds
-
The complete chloroplast genome sequence of an invasive plant, Tragopogon dubius Scopoli (asteraceae)
作者:Huang, Yue;Li, Jiamei;Huang, Yue;Cao, Jingjing;Zhao, Mengxin;Guo, Jianying;Wang, Rui;Wang, Rui;Li, Jiamei
关键词:Tragopogon dubius; complete chloroplast genome; phylogenetic analysis
-
Converting Tobacco Stalk Wastes into Value-Added Products via Sequential Hydrothermal and Pyrolysis Treatments
作者:Lin, Yingnan;Yu, Guofeng;Kong, Fanyu;Song, Dean;Liang, Rongning
关键词:biomass utilization; stepwise treatment; carbohydrate; aromatics; biochar
-
Flow Field Characteristics of Multi-Trophic Artificial Reef Based on Computation Fluid Dynamics
作者:Huang, Junlin;Li, Yan;Xia, Xu;Li, Jiao;Gong, Pihai;Guan, Changtao;Li, Jiao;Gong, Pihai;Guan, Changtao
关键词:artificial reef; flow field characteristics; computation fluid dynamics; multi-trophic structure
-
Characterisation of aroma compounds, sensory characteristics, and bioactive components of a new type of huangjiu fermented with Chinese wild rice (Zizania latifolia)
作者:Zhao, Yuzong;Gu, Mingyue;Fang, Song;Yan, Ning;Kong, Fanyu;Pang, Xueli;Qiu, Jun;Jiang, Peng;Ma, Donglin;Ren, Dongliang
关键词:Chinese wild rice ( Zizania latifolia ); Huangjiu; Aroma compounds; Overall quality; Sensory analysis; Bioactive components
-
OsmiR319-OsPCF5 modulate resistance to brown planthopper in rice through association with MYB proteins
作者:Sun, Bo;Shen, Yanjie;Zhu, Lin;Yang, Xiaofang;Miao, Xuexia;Shi, Zhenying;Sun, Bo;Shen, Yanjie;Zhu, Lin;Yang, Xiaofang;Liu, Xue;Li, Dayong;Zhu, Mulan
关键词:OsmiR319; Brown planthopper; TCP; MYB; Resistance