Rapid and sensitive detection of quizalofop-p-ethyl by gold nanoparticle-based lateral flow immunoassay in agriproducts and environmental samples

文献类型： 外文期刊

第一作者： Liu, Xue

作者： Liu, Xue;Kong, Fanyu;Qiu, Jun;Fang, Song;Li, Jiao;Huang, Yue;Zhang, Zhongrong;Hua, Xiude;Lin, Qing;Xia, Pengliang

作者机构：

关键词： Quizalofop-p-ethyl; Monoclonal antibody; Enzyme-linked immunosorbent assay; Gold nanoparticle-based lateral flow; immunoassay; Pesticide residue

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：9.8； 五年影响因子：9.6 ）

ISSN： 0048-9697

年卷期： 2023 年 857 卷

页码：

收录情况： SCI

摘要： Quizalofop-p-ethyl is a widely used herbicide that poses a threat to human health and environmental safety. In this study, anti-quizalofop-p-ethyl monoclonal antibodies (mAbs) were prepared and used to develop a gold nanoparticle-based lat-eral flow immunoassay (AuNP-LFIA) for the detection of quizalofop-p-ethyl in agriproducts and environmental samples. Four hybridoma cell lines were obtained, among which 5B6D10E11 secreted mAb with the highest sensitivity, with a 50 % inhibition concentration of 4.57 ng/mL in the indirect competitive enzyme-linked immunosorbent assay. After optimization, the AuNP-LFIA strip based on the mAb (5B6D10E11) showed a visual detection limit of 10 ng/mL, and the results could be directly determined by the naked eye within 8 min. The cross-reactivity of the AuNP-LFIA for analogs of quizalofop-p-ethyl was negligible except for quizalofop-p-acid. The established AuNP-LFIA was proven to be accurate and precise based on the recovery test. Furthermore, the detection results of AuNP-LFIA were consistent with those of ultra-high-performance liquid chromatography tandem mass spectrometry.

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