Development of CRISPR_Cas9 genome editing system and its application in rice molecular breeding
文献类型： 外文期刊
第一作者： Ma, Zengfeng
作者： Ma, Zengfeng;Wei, Minyi;Zhang, Yuexiong;Qin, Gang;Liu, Chi;Li, Zhenjing;Huang, Dahui
作者机构：
关键词： CRISPR; Cas9 system; Genome editing; Molecular breeding
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）
ISSN： 1054-5476
年卷期： 2021 年 57 卷 4 期
页码：
收录情况： SCI
摘要： Gene editing is a new genetic engineering technology that uses sequence-specific nucleases to insert, delete, or replace nucleic acid base(s) of the target gene, or introduce exogenous DNA sequences at a specific site in the genome of an organism to achieve the desired modification. Currently, the CRISPR/Cas system is the most widely used genome editing technology because it is relatively simple with high editing efficiency. In this review, we discuss the CRISPR/Cas system's application and its current limitations in rice breeding. Also, we elaborate on possible improved methods regarding its mechanism and development history.
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