Facile and green synthesis of reduced graphene oxide/loofah sponge for Streptomyces albulus immobilization and e-poly-L-lysine production

文献类型： 外文期刊

第一作者： Wang, Cheng

作者： Wang, Cheng;Li, Na;Xu, Hong;Zhu, Ping;Jiang, Yingying;Chen, Xi

作者机构：

关键词： Graphene oxide; Loofah sponge; Cell immobilization; Streptomyces albulus; e-Poly-L-lysine; Aerobic plant fibrous-bed bioreactor

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.889； 五年影响因子：11.139 ）

ISSN： 0960-8524

年卷期： 2022 年 349 卷

页码：

收录情况： SCI

摘要： Facile and green fabrication of reduced graphene oxide on loofah sponge (rGOLS) carrier was applied for cell immobilization of e-Poly -L-lysine (epsilon-PL) production. Due to surface properties including large specific surface area, high polarity, and low interaction energy, rGOLS-1 was employed as the optimum rGOLS to enhance immobilization of Streptomyces albulus. Compared with raw LS, batch experiments showed rGOLS-1 facilitated superior cell vitality for epsilon-PL production due to the presence of reduced graphene oxide. In the sequential fed batch cultivation of Streptomyces albulus using rGOLS-1 with an aerobic plant fibrous-bed bioreactor (rGOLS-1AFPB), the maximum epsilon-PL concentration and productivity reached to 39.2 +/- 0.63 g/L and 0.48 g/L/h. The cells immobilized in rGOLS-1 with high vitality and epsilon-PL production efficiency were reused six times over a period of 624 h. This research afforded an effective approach to enhance the fermentation performance of immobilized cells with the design of an advanced immobilization carrier.

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