Antigenic analysis monoclonal antibodies against different epitopes of sigma B protein of Muscovy duck reovirus

文献类型： 外文期刊

第一作者： Li, Yongfeng

作者： Li, Yongfeng;Yin, Xiuchen;Chen, Xiaodan;Li, Xiaojun;Li, Jinzhe;Liu, Chunguo;Liu, Ming;Zhang, Yun

作者机构：

关键词： Muscovy duck reovirus (DRV);sigma B protein;Pepscan;Epitope

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN： 0168-1702

年卷期： 2012 年 163 卷 2 期

页码：

收录情况： SCI

摘要： sigma B is one of the major structural proteins of Muscovy duck reovirus (DRV), which is able to induce protective immune response in target birds. Four anti-DRV sigma B MAbs were identified belong to two distinct epitopes, designated A (1E5, 4E3, and 5D8) and B (2F7) (Liu et al., 2010). To understand antigenic determinants of the sigma B protein, a set of 20 (P1-P20), partially overlapping and consecutive peptides spanning sigma B were expressed and then screened by MAbs. With Western blot and enzyme-linked immunosorbent assay (ELISA), two minimal units of the linear epitopes, 19YIRAPACWD27 (epitope B) and 65TDGVCF-PHHK74 (epitope A), were identified within N-terminal region of the sigma B protein. The epitope B was highly conserved among DRV and avian reovirus (ARV) strains through sequence alignment analysis. Immunofluorescence assays (IFA) and ELISA, confirmed that epitope B is a broad group-specific epitope among DRV and ARV. Epitope A could only react with chicken embyonated fibroblast cells (CEF) infected with DRV, but not ARV. However, both peptides have good immunogenicity and could induce antibodies against DRV in BALB/c mice. This report documents the first identification of sigma B epitopes in the precise locations. The two probes would be useful in the development of discriminating diagnostic kits for DRV and ARV infection. (C) 2011 Published by Elsevier B.V.

分类号：

  • 相关文献

[1]Antigenic structure analysis of VP3 of infectious bursal disease virus. Deng, Xiaoyun,Gao, Yulong,Gao, Honglei,Qi, Xiaole,Cheng, Yu,Wang, Xiaoyan,Wang, Xiaomei. 2007

[2]Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon alpha in mice and protective efficacy in guinea pigs and swine. Du, Yijun,Li, Yufeng,Jiang, Wenming,Wang, Xinglong,Tang, Bo,Cao, Jun,Wang, Xianwei,Jian, Ping,He, Hairong,Qi, Jing. 2008

[3]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[4]Anti-human fibroblast growth factor-21 monoclonal antibody preparation, characterization and analysis of in vitro bioactivity. Ding, Liangjun,Yuan, Qingyan,Xu, Pengfei,Yu, Yinhang,Li, Deshan,Hao, Zhichao. 2016

[5]Identification of two antigenic epitopes on SARS-CoV spike protein. Hua, RH,Zhou, YJ,Wang, YF,Hua, YZ,Tong, GZ. 2004

[6]Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Li, Xiaofei,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Wang, Xiaomei,Li, Xiaofei,Qin, Liting,Sun, Yingjun,Cui, Xuezhi,Gao, Yadong,Zhu, Haibo.

[7]SPIKE PROTEIN REGION (aa 636-789) OF PORCINE EPIDEMIC DIARRHEA VIRUS IS ESSENTIAL FOR INDUCTION OF NEUTRALIZING ANTIBODIES. Sun, D. B.,Feng, L.,Shi, H. Y.,Chen, J. F.,Liu, S. W.,Chen, H. Y.,Wang, Y. F..

[8]Monoclonal antibodies and conserved antigenic epitopes in the C terminus of GP5 protein of the North American type porcine reproductive and respiratory syndrome virus. Zhou, Yan-Jun,Yu, Hai,Li, Guo-Xin,Jiang, Yi-Feng,Tong, Guang-Zhi,Tian, Zhi-Jun,Liu, Jin-Xia,An, Tong-Qing,Peng, Jin-Mei,Cai, Xue-Hui,Wang, Mei,Wang, Yun-Feng,Xue, Qiang.

[9]Identifying Novel B Cell Epitopes within Toxoplasma gondii GRA6. Wang, Yanhua,Wang, Guangxiang,Cai, Jian Ping. 2016

[10]Phage display identifies an Eastern equine encephalitis virus glycoprotein E2-specific B cell epitope. Zhao, J.,Sun, E. C.,Liu, N. H.,Yang, T.,Xu, Q. Y.,Qin, Y. L.,Wu, D. L.,Zhao, J.,Sun, E. C.,Qin, Y. L.,Yang, Y. H.. 2012

[11]Screening and identification of novel B cell epitopes of Toxoplasma gondii SAG1. Wang, Yanhua,Wang, Guangxiang,Zhang, Delin,Yin, Hong,Zhang, Delin,Wang, Meng,Wang, Yanhua,Wang, Guangxiang,Zhang, Delin,Yin, Hong,Wang, Meng. 2013

[12]Critical Amino Acids Analysis of Epitopes in Pen a1. Mou Hui,Gao Mei-Xu,Wang Zhi-Dong,Zhao Jie,Li Shu-Jin,Zhao Xin,Li Shu-Rong,Pan Jia-Rong,Zhi Yu-Xiang. 2014

[13]Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus. Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Lei, Zhi-Hai,Zeng, Jiang-Yong,Zhu, Se,Duoji, Ciren,Dunzhu, Luobu. 2013

[14]Genotype-specific acquisition, evolution and adaptation of characteristic mutations in hepatitis E virus. Ikram, Aqsa,Hakim, Mohamad S.,Zhou, Jian-hua,Wang, Wenshi,Peppelenbosch, Maikel P.,Pan, Qiuwei,Ikram, Aqsa,Hakim, Mohamad S.,Zhou, Jian-hua. 2018

[15]Production and characterization of mouse monoclonal antibodies against lysis protein E of phiX174. Yu, Shenye,Zhao, Hailing,Wang, Huanan,Wang, Xiumei,Xu, Lili,Si, Wei,Chen, Liping,Zhang, Wanjiang,Liu, Siguo,Shao, Guangxi. 2013

[16]In silico analysis and recombinant expression of BamA protein as a universal vaccine against Escherichia coli in mice. Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua,Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua.

[17]Identification of a conserved B-cell epitope in the equine arteritis virus (EAV) N protein using the pepscan technique. Zhao, Shihua,Qi, Ting,Guo, Wei,Lu, Gang,Xiang, Wenhua.

[18]VP1 B-C and D-E loops of bovine enterovirus cluster B can effectively display foot-and-mouth disease virus type O-conserved neutralizing epitope. Chang, Jitao,Li, Yingli,Yang, Decheng,Wang, Fang,Jiang, Zhigang,Yu, Li.

[19]Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Ren, Jian-Le,Zhu, Yuan-Mao,Zhou, Yue-Hui,Lv, Chuang,Yan, Hao,Ma, Lei,Shi, Hong-Fei,Xue, Fei.

[20]Expression and antigenic epitopes mapping of receptor binding domain on the spike protein of severe acute respiratory syndrome coronavirus. Rua, RH,Tong, GZ,Wang, YF,Zhou, YJ. 2005

作者其他论文 更多>>

微信小程序