DRAMATICALLY REDUCED VIRULENCE OF MUTANTS OF PSEUDOMONAS-SOLANACEARUM DEFECTIVE IN EXPORT OF EXTRACELLULAR PROTEINS ACROSS THE OUTER-MEMBRANE

文献类型： 外文期刊

第一作者： KANG, YW

作者： KANG, YW;HUANG, JZ;MAO, GZ;HE, LY;SCHELL, MA

作者机构：

关键词： ENDOGLUCANASE;OUT GENES;PECTIN METHYLESTERASE;POLYGALACTURONASE;WILT DISEASE

期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS （ 影响因子：4.171； 五年影响因子：4.836 ）

ISSN： 0894-0282

年卷期： 1994 年 7 卷 3 期

页码：

收录情况： SCI

摘要： Pseudomonas solanacearum is an important wilt-inducing pathogen that infects a wide variety of important crop plants throughout the world. Studies using artificial inoculation methods suggest that some of its extracellular proteins play a significant, but auxiliary role in production of wilt disease. We isolated mutants of race 1 and race 3 strains of P. solanacearum with Tn5 insertions at a single locus (eep) whose culture supernatants lack all of its known extracellular enzymes and most other detectable extracellular proteins (EXPs). Analysis of subcellular fractions of eep::Tn5 mutants showed that they still synthesized many of these EXPs but accumulated them inside the cell. Experiments with PhoA fusion proteins showed that export of proteins across the inner membrane was not affected by the eep mutation, suggesting that eep functions only in protein export across the outer membrane. Production of extracellular polysaccharide was not obviously affected by the eep mutation. Analysis of eep mutants in stem-inoculated tomato plants showed that they had lost the ability to cause wilt symptoms or kill the plant, possibly because they colonized stems much more slowly than wild types. Plants grown in soil inoculated with the eep mutants did not develop any visible disease symptoms over a 20-day period, and their stems contained fewer than 10(3) P. solanacearum cells, whereas wild types killed plants in 14 days, and more than 10(10) cells were found in their stems. These results suggest that an individual or group of extracellular proteins of P. solanacearum is required for infection via the roots, as well as wilting and killing of host plants.

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