H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens

文献类型: 外文期刊

第一作者: Li, Chengjun

作者: Li, Chengjun;Ping, Jihui;Jing, Bo;Deng, Guohua;Jiang, Yongping;Li, Yanbing;Tian, Guobin;Yu, Kangzhen;Bu, Zhigao;Chen, Hualan

作者机构:

关键词: avian influenza;H5N1;marker vaccine

期刊名称:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( 影响因子:3.575; 五年影响因子:3.381 )

ISSN: 0006-291X

年卷期: 2008 年 372 卷 2 期

页码:

收录情况: SCI

摘要: Using plasmid-based reverse genetics, we generated a molecularly altered virus, H5N1/PR8-5B19, containing modified HA and NA genes from A/Goose/Guangdong/1/96 (GS/GD/1/96). In the H5N1/PR8-5B19 virus, the HA cleavage site was modified to resemble that of low-pathogenic avian strains and a portion of the NA stalk region was replaced by the immunodominant 51319 epitope of the S2 glycoprotein of murine hepatitis virus (MHV). H5N1/PR8-5B19 is not lethal to embryonated eggs or chickens. Chickens immunized with the H5N1/PR8-5B19 inactivated vaccine produced high levels of HI antibody and a measurable antibody response against the MHV 51319 epitope, and were fully protected against subsequent challenge with different highly pathogenic H5N1 avian influenza viruses. H5N1/PR8-5B19 is therefore an attractive marker vaccine candidate, eliciting a strong, protective antibody response and enabling serological discrimination between vaccinated and wild-type virus-infected chickens. (C) 2008 Elsevier Inc. All rights reserved.

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