Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFN gamma
文献类型: 外文期刊
第一作者: Xue, QN
作者: Xue, QN;Zhao, YG;Zhou, YJ;Qiu, HJ;Wang, YF;Wu, DL;Tian, ZJ;Tong, GZ
作者机构:
关键词: porcine reproductive and respiratory syndrome virus (PRRSV);DNA vaccine;porcine IL-2;porcine interferon
期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 影响因子:2.046; 五年影响因子:2.217 )
ISSN: 0165-2427
年卷期: 2004 年 102 卷 3 期
页码:
收录情况: SCI
摘要: In the present study, five eukaryotic double-gene expression plasmids containing porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 and ORF7 genes combined with cDNAs encoding porcine IFNgamma and IL-2 were constructed for evaluation as PRRSV vaccine candidates. After immunization and viral challenge, two of three pigs immunized with pIRESorf5/ IFNgamma, one of three pigs immunized with pIRESorf5/IL-2 and one of three pigs immunized with pIRESorf7IL-2 were protected from lung lesions that were present in other vaccinated and control animals. Virus replication was reduced but not completely prevented in organs of the DNA-vaccinated animals as compared to controls. Therefore, the porcine cytokines IFNgamma and IL-2, delivered in combination with ORF5 or ORF7, may improve the immune efficacy of DNA vaccines against PRRSV. (C) 2004 Elsevier B.V. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge
作者:Tian, ZJ;Zhou, GH;Zheng, BL;Qiu, HJ;Ni, JQ;Yang, HL;Yin, XN;Hu, SP;Tong, GZ
关键词:pseudorabies virus;swine influenza virus;recombinant virus;H3N2 subtype
-
Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus
作者:Zhou, YJ;An, TQ;He, YX;Liu, JX;Qiu, HJ;Wang, YF;Tong, G
关键词:PRRSV;GP3;epitope
-
Genetic diversity of avian infectious bronchitis coronavirus strains isolated in China between 1995 and 2004
作者:Liu, SW;Zhang, QX;Chen, JD;Han, ZX;Liu, X;Feng, L;Shao, YH;Rong, JG;Kong, XG;Tong, GZ
关键词:
-
RAPD profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China
作者:Chen, SL;Xia, T;Chen, SY;Zhou, YJ
关键词:Coelonema;genetic variation;RAPD;Qinghai-Tibet Plateau
-
Identification and antigenic epitope mapping of immunodominant region amino residues 510 to 672 on the spike protein of the severe acute respiratory syndrome coronavirus
作者:Hua, RH;Wang, YF;Bu, ZG;Zhou, YJ;Ge, JY;Wang, XJ;Tong, GZ
关键词:
-
Expression and antigenic epitopes mapping of receptor binding domain on the spike protein of severe acute respiratory syndrome coronavirus
作者:Rua, RH;Tong, GZ;Wang, YF;Zhou, YJ
关键词:severe acute respiratory syndrome (SARS-CoV);spike protein;epitope
-
Polymorphism detection of porcine PSMC3, PSMC6 and PSMD3 genes and their association with partial growth, carcass traits, meat quality and immune traits
作者:Wang, YF;Yang, J;Yu, M;Liu, B;Fan, B;Zhu, MJ;Xiong, IA;Li, K
关键词:porcine;PSMC3;PSMC6;PSMD3;polymorphism;association analysis