Immune responses of swine following DNA immunization with plasmids encoding porcine reproductive and respiratory syndrome virus ORFs 5 and 7, and porcine IL-2 and IFN gamma

文献类型: 外文期刊

第一作者: Xue, QN

作者: Xue, QN;Zhao, YG;Zhou, YJ;Qiu, HJ;Wang, YF;Wu, DL;Tian, ZJ;Tong, GZ

作者机构:

关键词: porcine reproductive and respiratory syndrome virus (PRRSV);DNA vaccine;porcine IL-2;porcine interferon

期刊名称:VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY ( 影响因子:2.046; 五年影响因子:2.217 )

ISSN: 0165-2427

年卷期: 2004 年 102 卷 3 期

页码:

收录情况: SCI

摘要: In the present study, five eukaryotic double-gene expression plasmids containing porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 and ORF7 genes combined with cDNAs encoding porcine IFNgamma and IL-2 were constructed for evaluation as PRRSV vaccine candidates. After immunization and viral challenge, two of three pigs immunized with pIRESorf5/ IFNgamma, one of three pigs immunized with pIRESorf5/IL-2 and one of three pigs immunized with pIRESorf7IL-2 were protected from lung lesions that were present in other vaccinated and control animals. Virus replication was reduced but not completely prevented in organs of the DNA-vaccinated animals as compared to controls. Therefore, the porcine cytokines IFNgamma and IL-2, delivered in combination with ORF5 or ORF7, may improve the immune efficacy of DNA vaccines against PRRSV. (C) 2004 Elsevier B.V. All rights reserved.

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