Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis

文献类型： 外文期刊

第一作者： Li, Xiangdong

作者： Li, Xiangdong;Qiu, Yafeng;Shen, Yang;Ding, Chan;Liu, Peihong;Zhou, Jinping;Ma, Zhiyong

作者机构：

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN： 0003-2697

年卷期： 2008 年 373 卷 2 期

页码：

收录情况： SCI

摘要： A modified polymerase chain reaction (PCR)-based site-directed mutagenesis method used to splice together different regions of a gene by deleting hundreds of nucleotides of undesired sequences is described. This method was inspired by a PCR-based site-directed mutagenesis method developed by Stratagene (La Jolla, CA, USA); the procedure and primer design were modified to enable the method to generate deletions several hundreds of nucleotides in length with an efficiency of 80-100%, and to delete two DNA fragments simultaneously in a single PCR. This method should be useful for deletion of large DNA fragments from a gene. (C) 2007 Elsevier Inc. All rights reserved.

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