Real-Time Detection of LAMP Products of African Swine Fever Virus Using Fluorescence and Surface Plasmon Resonance Method

文献类型： 外文期刊

第一作者： Zhang, Hao

作者： Zhang, Hao;Yao, Yuan;Qiu, Xianbo;Yu, Duli;Zhang, Lulu;Chen, Zhi;Sun, Wenbo;Liu, Xiang;Sun, Jianhai;Chen, Lei

作者机构：

关键词： African swine fever virus (ASFV); loop-mediated isothermal amplification (LAMP); surface plasmon resonance (SPR); fluorescence detection

期刊名称：BIOSENSORS-BASEL （ 影响因子：5.519； 五年影响因子：5.313 ）

ISSN：

年卷期： 2022 年 12 卷 4 期

页码：

收录情况： SCI

摘要： African swine fever (ASF) is a swine disease with a very high fatality rate caused by a complex double-stranded DNA virus. The fluorescence PCR detection method is widely used for virus nucleic acid detection. Surface plasmon resonance (SPR) is a label-free and real-time detection method, unlike the fluorescence PCR detection method. In this research, we detected the loop-mediated isothermal amplification (LAMP) products of the African swine fever virus by using the SPR and fluorescence methods separately and simultaneously. By comparing the positive and negative control results, we found that the SPR response unit is completely different before and after the LAMP process. In addition, the fluorescence results on a chip showed that with an increase in the concentration of the sample, the cycle threshold (CT) value decreased, which is consistent with commercial instruments. Both the decline rate of the SPR response unit and the CT value of the fluorescence realized were used to distinguish the positive control from the negative control and water, which indicates that the SPR method can be combined with fluorescence to detect LAMP products. This research provides a label-free and simple method for detecting LAMP products.

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