Three Novel Adenylate Cyclase Genes Show Significant Biological Functions in Plant

文献类型： 外文期刊

第一作者： Liu, Zhiguo

作者： Liu, Zhiguo;Wang, Lili;Zhao, Xuan;Zhao, Zhihui;Liu, Mengjun;Liu, Zhiguo;Yuan, Ye;Wang, Lixin;Zhao, Xin;Chu, Yuetong;Yang, Fangyuan;Wang, Yulu;Zhao, Jin;Liu, Mengjun;Liu, Zhiguo;Liu, Mengjun;Zhao, Xuan;Wang, Lihu;Zhang, Qiong

作者机构：

关键词： adenylate cyclase; cyclic adenosine monophosphate; Chinese jujube; biological function; regulation mechanism

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 2 期

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收录情况： SCI

摘要： Adenylate cyclase is the key enzyme solely synthesizing cAMP which participates in cell metabolism regulations and functions as an intracellular second messenger. However, the biological functions of plant ACs have not been elucidated clearly for their poor conservative sequences and low detectable cAMP. We performed a systematic study of plant ACs by using Chinese jujube, whose fruit exhibits the highest cAMP content among plants. Three novel ACs were identified from Chinese jujube, and two types of methods including in vitro and in vivo were used to certificate ZjAC1-3 which can catalyze the conversion of ATP into cAMP. The biological functions of significant accelerations of seed germination, root growth, and flowering were found via overexpression of these AC genes in Arabidopsis, and these functions of ACs were further demonstrated by treating the AC-overexpressing transgenic lines and wild type Arabidopsis with bithionol and dibutyryl-cAMP. At last, transcriptome data revealed that the underlying mechanism of the biological functions of ACs might be regulation of the key genes involved in the circadian rhythm pathway and the hormone signal transduction pathway. This research established a foundation for further investigating plant AC genes and provided strong evidence for cAMP serving as a signaling molecule in plants.

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