Enhancing wuyiencin productivity of Streptomyces albulus (CK15) by mutagenesis breeding with atmospheric and room temperature plasma

文献类型： 外文期刊

第一作者： Thein, Yu Wah

作者： Thein, Yu Wah;Shi, Liming;Liu, Binghua;Wei, Qiuhe;Zhang, Kecheng;Ge, Beibei;Thein, Yu Wah

作者机构：

关键词： Streptomyces albulus; Wuyiencin; Atmospheric and room temperature plasma (ARTP)

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：4.1； 五年影响因子：4.2 ）

ISSN： 0959-3993

年卷期： 2023 年 39 卷 8 期

页码：

收录情况： SCI

摘要： Streptomyces species are known for their ability to efficiently produce secondary metabolites, including various antibiotics. Wuyiencin, an antibiotic produced by Streptomyces albulus CK15, is commonly used in agriculture to control fungal diseases in crops and vegetables. In this study, we utilized atmospheric and room temperature plasma (ARTP) mutagenesis to generate mutant S. albulus strains with improved fermentation capabilities for wuyiencin production. After mutagenizing the wild-type S. albulus CK15 strain once and conducting two rounds of antimicrobial screening, three genetically stable mutants (M19, M26, and M28) were identified. These mutants showed increased wuyiencin production by 17.4%, 13.6%, and 18.5% in comparison to the CK15 strain in flask culture, respectively. The M28 mutant exhibited the highest wuyiencin activity, producing 1443.0 +/- 134.6 U/mL in flask culture and 1673.8 +/- 127.4 U/mL in a 5 L fermenter. These results demonstrate that ARTP is an efficient tool for microbial mutation breeding and improving wuyiencin production.

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