Comparative physiological and cytological analysis, and omics approach provide clues to the coloring mechanism of the pumpkin yellow stems

文献类型： 外文期刊

第一作者： Deng, Liting

作者： Deng, Liting;Luo, Jianning;Wu, Haibin;Liu, Xiaoxi;Zhao, Gangjun;Gong, Hao;Zheng, Xiaoming;Ni, Chaoqun;Wang, Xueting;Li, Junxing;Ni, Chaoqun

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关键词： Pumpkin; Chlorisis; Chloroplast; Chlorophyll; Carotenoid; Photosynthesis

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）

ISSN： 1471-2229

年卷期： 2025 年 25 卷 1 期

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收录情况： SCI

摘要： BackgroundThe abnormal chloroplast and pigment accumulation could lead to plant yellowing. However, the plant tissue chlorisis is species-specific and could display various phenotypes due to the genetic and environmental impacts. The molecular mechanisms underlying the plant stem yellowing are less understood than the flower or leaf coloring mechanisms. Herein, the physiological, cytological, transcriptome analysis, along with genome-wide association study (GWAS) were integrated to illustrate the processes relevant to pumpkin stem coloring.ResultsSimilar yet different variations were discovered in the pumpkin yellow stems. Low content of photosynthetic pigments, and impaired chloroplast thylakoid membrane were identified in the pumpkin yellowing stems, together with the presence of plastoglobules and starch grains. Elevated expression of genes in catabolism of chlorophylls and carotenoids was found in yellow stems, which may result in the failed accumulation of pigments and pumpkin stem chlorisis. Concurrently, increased expression of genes in chloroplast development, antioxidant protection, photosynthesis, and ribosome were found, which may act as compensation mechanisms for chloroplast defects. The integrated analysis of transcriptome and GWAS identified the up-regulated proteases and decreased kinesins in yellow stems, which could result in the breakdown of thylakoid systems, and the disability of photosynthetic pigments accumulation. Additionally, transcription factors could be involved in the regulation of the specific color change in pumpkin stems.ConclusionsThese findings provide clues into the molecular mechanisms of stem yellowing, and will facilitate the exploration of candidate targets as markers or genetic improvement through molecular breeding.

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