Uncovering expression and functional analysis of newly discovered high-affinity K+ transporter family members from sugarcane

文献类型： 外文期刊

第一作者： Luo, Hai-bin

作者： Luo, Hai-bin;Huang, Cheng-mei;Wei, Yuan-wen;Cao, Hui-qing;Jiang, Sheng-li;Zhu, Hui-ming;Deng, Zhi-nian;Wu, Kai-chao;Xu, Lin

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关键词： Sugarcane; High-affinity K+ transporter genes(HAK); Gene cloning; Gene function; Gene expression

期刊名称：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：1.175； 五年影响因子：1.238 ）

ISSN： 0971-7811

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收录情况： SCI

摘要： Potassium ions are widely involved in a series of physiological and biochemical processes of plants, which is of great significance to plant growth. High-affinity K+ (HAK) transporter mainly absorbed and transported potassium in plants, but there are few studies on HAK gene in sugarcane. In this study, the coding region of high-affinity K+ transporter genes were cloned from sugarcane and designated as ScHAK9, ScHAK10, ScHAK11 (GenBank accession number: MG564720, MG564721, MG564722). Phylogenetic analysis results confirmed that ScHAK9 and ScHAK11 had the closest relationship with ZmHAK9 and ZmHAK11(Zea mays), ScHAK10 had the closest relationship with SbHAK10 (Sorghum bicolor). In subcellular localization experiments, the fusion protein of ScHAK9 and ScHAK11 with green fluorescent protein was specifically localized in the cell membrane, but ScHAK10 green fluorescent protein was not detected, it was speculated to be expressed in Golgi apparatus. The gene expression level of ScHAK in different tissues of sugarcane at the growth periods was different, and the gene expression level of ScHAK genes were up-regulated by the low-potassium and salt stress. Through the functional characterization experiments of ScHAK genes in K+ uptake-deficient yeasts, it was founded that ScHAK genes possessed K+ transporter activity. The study indicated that ScHAK genes might mediate K+ absorption through the cell membrane and might be participate in maintaining Na+/K+ homeostasis in sugarcane under the adversity stress, and the development of plant organs is regulated by the potassium ions transport of ScHAK genes.

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