Phase separation of CgSCE3 is required for heat stress resistance and pathogenicity of Colletotrichum gloeosporioides

文献类型： 外文期刊

第一作者： Zhao, Zhihao

作者： Zhao, Zhihao;Zhang, Yi;Luo, Hongli;An, Bang;Wang, Qiannan;Zhao, Zhihao;Zhang, Yi;Luo, Hongli;An, Bang;Wang, Qiannan;Xie, Qingbiao

作者机构：

关键词： CgSCE3; Phase separation; Heat stress; Stress granule; Pathogenicity; Colletotrichum gloeosporioides

期刊名称：MICROBIOLOGICAL RESEARCH （ 影响因子：6.9； 五年影响因子：7.2 ）

ISSN： 0944-5013

年卷期： 2025 年 300 卷

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收录情况： SCI

摘要： Temperature fluctuations can significantly impact molecular interactions and biochemical reactions essential for cellular homeostasis. For organisms unable to regulate their own temperature, adaptation to unpredictable thermal stress remains a significant challenge. Biomolecular condensation has emerged as a potential mechanism for encoding thermal responsiveness and ensuring biochemical robustness. However, it remains unclear whether proteins in plant-pathogenic fungi undergo phase separation in response to heat stress and whether this process contributes to host infection. Here, we investigate thermal adaptation in the filamentous fungus Colletotrichum gloeosporioides, a major pathogen responsible for anthracnose, which causes significant economic losses in rubber tree and other tropical crops. We identify CgSCE3, an RNA-binding protein homologue highly expressed during both appressoria formation and invasive hyphal growth in C. gloeosporioides. CgSCE3 contains a predicted RNA recognition motif (RRM) and three intrinsically disordered regions (IDRs). Strikingly, CgSCE3 was found to form phase-separated condensates in vivo under heat shock (HS) conditions. Domain deletion analyses demonstrate that all three IDRs and the RRM contribute to phase separation, with IDR2 playing a particularly crucial role. Additionally, CgSCE3 phase separation was shown to be essential for both thermotolerance and pathogenicity of C. gloeosporioides. Furthermore, CgSCE3 was also found to participate in the regulation of CgPab1-mediated stress granule formation during HS. Together, these results provide mechanistic insight into protein phase separation as a conserved heat stress response strategy in phytopathogenic fungi. More broadly, our findings suggest that manipulating protein phase separation could enhance fungal stress resilience and virulence, offering potential implications for agricultural disease management.

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