Establishment and Application of Multiplex PCR Systems Based on Molecular Markers for HMW-GSs in Wheat

文献类型： 外文期刊

第一作者： Yao, Chuxuan

作者： Yao, Chuxuan;Zhou, Shuo;Dong, Fushuang;Liu, Yongwei;Yang, Fan;Jiao, Bo;Zhao, He;Lyu, Mengyu;Wang, Haibo;Chai, Jianfang;Yao, Chuxuan;Bi, Caili;Zhang, Cuimian

作者机构：

关键词： Bx7(OE); multiplex PCR markers; HMW-GSs; wheat quality

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.408； 五年影响因子：3.459 ）

ISSN：

年卷期： 2022 年 12 卷 4 期

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收录情况： SCI

摘要： High-molecular-weight glutenin subunits (HMW-GSs) encoded by alleles at the Glu-A1, Glu-B1, and Glu-D1 loci confer unique end-use quality properties of common wheat (Triticum aestivum L.). Wheat accessions with the high-quality HMW-GSs combination of Ax2*/Bx7(OE)/Dx5 usually exhibit strong gluten characteristics. In order to stack these three high-quality subunit genes by molecular markers in strong gluten wheat breeding, an agarose gel-based multiplex PCR marker for these high-quality HMW-GSs and two agarose gel-based multiplex PCR markers detecting the homozygosity of Ax2* and Bx7(OE) subunits were developed. These markers were verified in an F-2 segregating population from a cross between a medium-gluten winter wheat cultivar with the HMW-GSs combination of Ax null/Bx7 + By8/Dx4 + Dy12 and a strong-gluten spring wheat cultivar with the HMW-GSs combination of Ax2*/Bx7(OE) + By8*/Dx5 + Dy10. By integrating the newly established multiplex PCR markers and a published co-dominant PCR marker of the Dx5 subunit, a complete molecular marker selection system was established. After multiple rounds of molecular marker-assisted selection with the system, 17 homozygous winter wheat lines that stacked the three high-quality HMW-GSs were generated. The gluten strength of these homozygous lines was comparable to their strong-gluten parent, but significantly higher than that of their medium-gluten parent by measuring their lactic acid-sodium dodecyl sulfate solvent retention capacities of whole wheat meal. The multiplex PCR systems established in the present study can be used for molecular marker-assisted selection of strong gluten wheats.

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