文献类型： 外文期刊
第一作者： Chen, Daozong
作者： Chen, Daozong;Liu, Yi;Jin, Qingdong;Pan, Jianming;Tang, Yijia;Yanrong, E.;Xu, Linshan;Yang, Taihua;Qiu, Jie;Chen, Xiaodi;Wang, Jing;Ge, Xianhong;Li, Zaiyun;Gong, Deping;Cui, Cheng
作者机构：
关键词： Brassica napus; Fine mapping; Flower color; Anthocyanins
期刊名称：MOLECULAR BREEDING （ 影响因子：3.1； 五年影响因子：3.1 ）
ISSN： 1380-3743
年卷期： 2023 年 43 卷 3 期
页码：
收录情况： SCI
摘要： Purple/red appearanceis one of the common phenotypic variations in leaves, stems, and siliques of oilseed rape (Brassica napus L.) but very rare in flowers. In this study, the causal genes for the purple/red traits in stems and flowers in two accessions of oilseed rape (DH_PR and DH_GC001, respectively) derived from the wide hybridization were fine mapped, and candidate genes were determined by methods combined with bulked segregant analysis (BSA) and RNA-seq analysis. Both traits of purple stem and red flowers were mapped to the locus as AtPAP2 homologous genes (BnaPAP2.C6a and BnaPAP2.A7b, respectively) belonging to the R2R3-MYB family. Sequence comparisons of full-length allelic genes revealed several InDels and SNPs in intron 1 as well as exons, and completely different promoter region of BnaPAP2.C6a and a 211 bp insertion was identified in the promoter region of BnaPAP2.A7b of DH_GC001. Our results not only contribute to a better understanding of anthocyanin inheritance in B. napus, but also provide a useful toolbox for future breeding of cultivars with purple/red traits through the combination of different functional alleles and homologs.
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