The alpha-Helical Cap Domain of a Novel Esterase from Gut Alistipes shahii Shaping the Substrate-Binding Pocket

文献类型： 外文期刊

第一作者： Wei, Xue

作者： Wei, Xue;Wang, Yu-lu;Wen, Bo-ting;Liu, Shu-jun;Wang, Luyao;Sun, Lichao;Gu, Tian-yi;Li, Zhen;Bao, Yuming;Wang, Fengzhong;Xin, Fengjiao;Fan, Shi-long;Zhou, Huan

作者机构：

关键词： ferulic acid esterase; gut microbiota; Alistipes shahii; crystal structure; cap domain

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2021 年 69 卷 21 期

页码：

收录情况： SCI

摘要： The human gut microbiota regulates nutritional metabolism, especially by encoding specific ferulic acid esterases (FAEs) to release functional ferulic acid (FA) from dietary fiber. In our previous study, we observed seven upregulated FAE genes during in vitro fecal slurry fermentation using wheat bran. Here, a 29 kDa FAE (AsFAE) from Alistipes shahii of Bacteroides was characterized and identified as the type-A FAE. The X-ray structure of AsFAE has been determined, revealing a unique a-helical domain comprising five a-helices, which was first characterized in FAEs from the gut microbiota. Further molecular docking analysis and biochemical studies revealed that Tyr100, Thr122, Tyr219, and Ile220 are essential for substrate binding and catalytic efficiency. Additionally, Glu129 and Lys130 in the cap domain shaped the substrate-binding pocket and affected the substrate preference. This is the first report on A. shahii FAE, providing a theoretical basis for the dietary metabolism in the human gut.

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