High-level production of pullulan from high concentration of glucose by mutagenesis and adaptive laboratory evolution of Aureobasidium pullulans

文献类型： 外文期刊

第一作者： Li, Xiwen

作者： Li, Xiwen;Yu, Xiaobin;Li, Xiwen;Zhao, Shuangzhi;Chen, Leilei;Zhou, Qingxin;Qiu, Jiying;Xin, Xue;Zhang, Yanhao;Yuan, Wei;Yang, Jinyu;Chen, Leilei;Zhou, Qingxin;Yuan, Wei;Tian, Chengsen;Chen, Leilei;Zhou, Qingxin;Yuan, Wei;Tian, Chengsen

作者机构：

关键词： Pullulan; Aureobasidium pullulans; Adaptive laboratory evolution; High glucose concentration

期刊名称：CARBOHYDRATE POLYMERS （ 影响因子：11.2； 五年影响因子：10.2 ）

ISSN： 0144-8617

年卷期： 2023 年 302 卷

页码：

收录情况： SCI

摘要： The cost of carbon sources and the low efficiency of the fermentation titer limit the industrial application of pullulan. In this study, a hypertonic-tolerant strain with efficient utilization of glucose was obtained using a double strategy. Initially, a strain for efficient synthesis of pullulan from glucose was generated by mutagenesis. Subsequently, the mutant was directionally evolved on the plate containing a high glucose concentration to enhance high osmotic resistance. The enzyme activities and the transcriptional levels involved in pullulan biosynthesis and high osmotic tolerance in mutants were increased. Nitrogen source and inorganic salts also significantly affected the production of pullulan by M233-20 from high concentration of glucose. The pullulan titer of 162.1 g/L was obtained using the response surface methodology in the flask. The strain M233-20 pro-duced 162.3 g/L pullulan in a 30-L bioreactor with a yield of 0.82 g/g glucose. Hence, this work provides a potential industrial pullulan producer M233-20 and a strategy to develop excellent strain.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序