Advanced gene editing techniques for enhancing disease resistance and climate resilience in crops

文献类型： 外文期刊

第一作者： Sarfraz, Zareen

作者： Sarfraz, Zareen;Iqbal, Muhammad Shahid;Zarlashat, Yusra;Ambreen, Alia;Mujahid, Muhammad

作者机构：

关键词： CRISPR-Cas9; disease resistance; gene editing; multi resistance; sustainable agriculture; TALENs; ZFNs; zinc finger

期刊名称：FUNCTIONAL PLANT BIOLOGY （ 影响因子：2.7； 五年影响因子：3.0 ）

ISSN： 1445-4408

年卷期： 2025 年 52 卷 6 期

页码：

收录情况： SCI

摘要： Ensuring food security and solving the issues brought on by climate change require breeding and engineering of climate-resilient crops. Despite its contributions to reducing agricultural diseases, genetic engineering has several limitations, including high labor costs, lengthy processing times, and poor productivity. Genome editing has become a potential method to provide notable opportunities to explain complex biological processes, genetically solve the causes of diseases, and improve crops for disease resistance by effectively modifying multiple traits. Genome editing techniques including TALENs, ZFNs, and CRISPR/Cas9 increase agricultural productivity by developing climate-resistant crops and promoting climate-resilient agriculture. Among these approaches, CRISPR/Cas9 shows exceptional efficacy, minimal chance of off-target effects, and improved traits such as drought tolerance and disease resistance. This study explores advanced gene editing techniques for improving disease resistance in crops and developing climate-resilient varieties to reduce food insecurity and hunger. It demonstrates that these techniques have enhanced the nutritional content and resilience of many crops by fighting abiotic and biotic stresses. Future agricultural practices could alter the genes and improve disease-resistant crops by genome editing techniques.

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