Isolation and Analysis of Cold Stress Inducible Genes in Zea mays by Suppression Subtractive Hybridization and cDNA Macroarray

文献类型： 外文期刊

第一作者： Zhang, Jinpeng

作者： Zhang, Jinpeng;Wang, Guoying;Zhang, Jinpeng;Wang, Guoying;Zhang, Ying;Fu, Junjie;Gu, Riliang;Wang, Jianhua;Chen, Xiaoping;Jia, Jinping;Zhang, Ying;Fu, Junjie;Gu, Riliang;Wang, Jianhua;Chen, Xiaoping;Jia, Jinping

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期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

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收录情况： SCI

摘要： In order to understand the molecular and cellular mechanisms underlying cold stress conditions (4pC) in maize seedlings, a forward subtractive cDNA library was constructed using the suppression subtractive hybridization (SSH) technique. Through the Virtual Northern blot analysis, 893 positive clones were screened from a total 1,200 clones in the subtractive cDNA library. After sequencing 528 randomly chosen cDNA clones, 213 uniquely expressed sequence tags (ESTs) were obtained by clustering and blast analysis, which included transcripts that had previously been reported as responsive to stress as well as some functionally unknown transcripts. Based on a list of functional Arabidopsis protein categories, the ESTs with significant protein similarity were sorted into ten functional categories. A cDNA macroarray containing the 213 unique ESTs was used to monitor the spatial and temporal distribution of gene expression in maize seedlings during cold stress. The results showed that 118 ESTs were induced by cold-stress in maize seedlings and 66 ESTs identified in the leaves and 89 ESTs in the roots. Hierarchical cluster analysis indicated that the expression profiles of cold stress inducible ESTs in the leaves were different from that observed in the roots. Moreover, some induced genes were related to sugar synthesis and reestablishment of high rates of photosynthesis. In addition, Northern blot analysis validated well the cDNA macroarray data.

分类号： Q946

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