Functional Characterization of the Nuclear Receptor Gene SaE75 in the Grain Aphid, Sitobion avenae

文献类型： 外文期刊

第一作者： Zheng, Haixia

作者： Zheng, Haixia;Yan, Yi;Wei, Guohua;Yan, Yi;Wei, Guohua;Gu, Yaxin;Zhu, Xun;Zhang, Yunhui;Li, Xiangrui;Merchant, Austin;Zhou, Xuguo;Gu, Yaxin

作者机构： Shanxi Agr Univ, Coll Plant Protect, Jinzhong 030800, Peoples R China;Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China;Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA;Tibet Agr & Anim Husb Univ, Coll Plant Sci, Linzhi 860000, Peoples R China

关键词： Sitobion avenae; SaE75; ecdysone signaling pathway; 20E; molting

期刊名称：INSECTS （ 2022影响因子：3.0； 五年影响因子：3.1 ）

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年卷期： 2023 年 14 卷 4 期

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收录情况： SCI

摘要： Simple Summary The English grain aphid, Sitobion avenae, is one of the major pests and disease vectors of wheat crops. In this study, E75, a key gene associated with ecdysone signal pathway, was identified and cloned from S. avenae. Functional characterization of E75 confirmed its role in the regulation of growth, development, and molting process of aphids. This finding offers a potential molecular target for the subsequent development of genetic-based biopesticides for aphid control. Ecdysteroid hormones are key regulators of insect development and metamorphosis. Ecdysone-inducible E75, a major component of insect ecdysone signaling pathway, has been well characterized in holometabolous insects, however, barely in hemimetabolous species. In this study, a total of four full-length E75 cDNAs from the English grain aphid, Sitobion avenae, were identified, cloned, and characterized. The four SaE75 cDNAs contained 3048, 2625, 2505, and 2179 bp open reading frames (ORF), encoding 1015, 874, 856, and 835 amino acids, respectively. Temporal expression profiles showed that SaE75 expression was low in adult stages, while high in pseudo embryo and nymphal stages. SaE75 was differentially expressed between winged and wingless morphs. RNAi-mediated suppression of SaE75 led to substantial biological impacts, including mortality and molting defects. As for the pleiotropic effects on downstream ecdysone pathway genes, SaHr3 (hormone receptor like in 46) was significantly up-regulated, while Sabr-c (broad-complex core protein gene) and Saftz-f1 (transcription factor 1) were significantly down-regulated. These combined results not only shed light on the regulatory role of E75 in the ecdysone signaling pathway, but also provide a potential novel target for the long-term sustainable management of S. avenae, a devastating global grain pest.

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