Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis

文献类型： 外文期刊

第一作者： Guo, Xiaola

作者： Guo, Xiaola;Wang, Shuai;Zhang, Junmei;Li, Rui;Zhang, Yong'e;Wang, Zhengrong;Kong, Qingming;Cho, William C.;Ju, Xianghong;Shen, Yujuan;Cao, Jianping;Zhang, Lingqiang;Fan, Haining;Zheng, Yadong;Zheng, Yadong

作者机构：

期刊名称：PLOS NEGLECTED TROPICAL DISEASES （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 1935-2735

年卷期： 2022 年 16 卷 10 期

页码：

收录情况： SCI

摘要： Echinococcosis is a parasitic disease caused by the metacestodes of Echinococcus spp. The disease has a long latent period and is largely underdiagnosed, partially because of the lack of effective early diagnostic approaches. Using liquid chromatography-mass spectrometry, we profiled the serum-derived extracellular vesicles (EVs) of E. multilocularis-infected mice and identified three parasite-origin proteins, thioredoxin peroxidase 1 (TPx-1), transitional endoplasmic reticulum ATPase (TER ATPase), and 14-3-3, being continuously released by the parasites into the sera during the infection via EVs. Using ELISA, both TPx-1 and TER ATPase were shown to have a good performance in diagnosis of experimental murine echinococcosis as early as 10 days post infection and of human echinococcosis compared with that of control. Moreover, TER ATPase and TPx-1 were further demonstrated to be suitable for evaluation of the prognosis of patients with treatment. The present study discovers the potential of TER ATPase and TPx-1 as promising diagnostic candidates for echinococcosis.

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