Catellatospora tritici sp. nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora

文献类型： 外文期刊

第一作者： Yang, Yanming

作者： Yang, Yanming;Qian, Lulu;Lin, Jiaying;Qi, Cuiping;Zhao, Tianxin;Wang, Xiangjing;Zhao, Junwei;Xiang, Wensheng;Xiang, Wensheng

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关键词： Catellatospora tritici sp. nov.; cellulase-producing actinobacterium; wheat rhizosphere soil; polyphasic approach; 16S rRNA gene; genome

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.689； 五年影响因子：2.925 ）

ISSN： 1466-5026

年卷期： 2022 年 72 卷 6 期

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收录情况： SCI

摘要： A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18(T), was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China. The novel strain was characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics confirmed that strain NEAU-YM18(T) belonged to the genus Catellatospora. Cells of strain NEAU-YM18(T) were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids. The acyl type of the cell-wall muramic acid was glycolyl. The whole-cell hydrolysates were xylose, glucose and ribose. The phospholipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were iso-C-15:0, iso-C-16:0, C-18:1 omega 9c and summed feature 5 (anteiso-C-18:0/C-18:2 omega 6,9c). The menaquinones were MK-9(H-4), MK-9(H-6) and MK-9(H-2). The DNA G+C content was 71.1 %. The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18(T) was closely related to Catellatospora chokoriensis 2-25(1)(T) (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505(T) (98.3 %) and formed a branch with C. sichuanensis H14505(T). Furthermore, the whole genome phylogeny of strain NEAU-YM18(T) showed that the strain formed an independent clade. The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)(T), C. vulcania NEAU-JM1(T) and C. sichuanensis H14505(T) were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively. These genetic results and some phenotypic characteristics could distinguish strain NEAU-YM18(T) from its reference strains. In addition, genomic analysis confirmed that strain NEAU-YM18(T) had the potential to decompose cellulose and produce bioactive compounds. Therefore, strain NEAU-YM18(T) represents a novel species of the genus Catellatospora, for which the name Catellatospora tritici sp. nov. is proposed. The type strain is NEAU-YM18(T) (= CCTCC AA 2020040(T)=JCM 33977(T)).

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