Transcriptomic analysis of Mythimna separata ovaries and identification of genes involved in reproduction

文献类型： 外文期刊

第一作者： Fu, Junhong

作者： Fu, Junhong;Hu, Chaoxing;Fu, Junhong;Li, Lingling;Dai, Changgeng;Zhang, Ying;Hu, Yang;Li, Hongbo

作者机构：

关键词： Mythimna separata; Transcriptome; Ovary development; Gene expression

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 1744-117X

年卷期： 2023 年 46 卷

页码：

收录情况： SCI

摘要： The migratory insect Mythimna separata is a major pest of grain crops in Asia. Unfortunately, the molecular mechanisms that control and regulate reproduction in this species remain unclear. In this study, transcriptome sequencing was utilized to identify genes associated with ovary development and oogenesis. Clean sequences totaling 117.71 Gb were assembled into 178,534 unigenes with a mean length of 647.37 bp and N50 length of 837 bp. Transcriptome analysis showed that 7921 unigenes were significantly expressed in ovaries with 4403 and 3518 unigenes up- and down-regulated, respectively. Enrichment analysis with the Kyoto Encyclopedia of Genes and Genomes database suggested that 729 differentially expressed genes were significantly enriched in the top 20 pathways (q-values <0.05). Twenty genes were associated with ovary development and oogenesis and included lipases, Nanos, small heat shock proteins (sHsps) and histones; these were further verified by qRT-PCR and may play essential roles in M. separata reproduction. Collectively, our findings reveal underlying mechanisms of M.separata reproduction and may lead to RNAi-based management strategies targeting reproductive physiology.

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