Rapid and reagent-free bioassay using autobioluminescent yeasts to detect agonistic and antagonistic activities of bisphenols against rat androgen receptor and progesterone receptor

文献类型： 外文期刊

第一作者： Huang, Yuan

作者： Huang, Yuan;Zhang, Wei;Cui, Na;Xiao, Zhiming;Wang, Ruiguo;Su, Xiaoou;Zhang, Chengdong

作者机构：

关键词： Steroid hormone receptors; Autobioluminescence; Yeast; In vitro; Bisphenols

期刊名称：JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：5.011； 五年影响因子：4.649 ）

ISSN： 0960-0760

年卷期： 2022 年 222 卷

页码：

收录情况： SCI

摘要： Bisphenol A (BPA) and its analogues have been classified as endocrine disruptors via binding to nuclear receptors. Two novel bioassays, BLYrARS and BLYrPRS, were developed for rapid detection of agonistic and antagonistic activities of BPA and five of its analogues binding rat androgen receptor (rAR) and rat progesterone receptor (rPR). The reporter bioassay was based on two autonomously bioluminescent strains of the yeast Saccharomyces cerevisiae, recombined with a bacterial luciferase reporter gene cassette (lux) that can produce autofluorescence, regulated by the corresponding hormone response element acting as the responsive promoter. The bioluminescent signal is autonomous and continuous without cell lysis or addition of exogenous reagents. The AR agonist R1881 could be detected at 4 h with a half-maximal effective concentration (EC50) of -9.4 nM. The PR agonist progesterone could be determined at 4 h with an EC50 of -2.74 nM. None of the sixteen bisphenols presented agonistic activities against rAR and rPR. However, thirteen BPs were rAR antagonists and eleven BPs acted as rPR antagonists with different potency. The BLYrARS and BLYrPRS bioassay characterized by automated signal acquisition without additional manipulations or cost can be applied for simple and rapid detection of agonistic and antagonistic activities of BPs and other compounds acting as agonists or antagonists of rAR and rPR. Based on data derived by use of this bioassay endocrine-disrupting activities of some BPA analogues are more potent than BPA.

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