Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole

文献类型： 外文期刊

第一作者： Zhang, Weikang

作者： Zhang, Weikang;Xu, Jiakun;Zhang, Weikang;Meng, Xiangmin;Lin, Yingwu;Wang, Qiaoning;Chen, Lingxin

作者机构：

关键词： Sulfonamides; Myoglobin; Biocatalysis; Biodegradation; Directed evolution

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2024 年 465 卷

页码：

收录情况： SCI

摘要： Sulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., kcat/Km = 720.42 M-1 s-1) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45 +/- 0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation

分类号：

  • 相关文献
作者其他论文 更多>>
  • A novel ZIF-L/PEI thin film nanocomposite membrane for removing perfluoroalkyl substances (PFASs) from water: Enhanced retention and high flux

    作者：Bi, Yujie;Meng, Xiangmin;Yong, Qiaozhi;Wang, Xinping;Tan, Zhijun;Geng, Qianqian;Peng, Jixing;Sun, Xiaojie;Guo, Mengmeng

    关键词：Perfluoroalkyl substances (PFASs); Thin film nanocomposite (TFN); Metal-organic framework (MOF); Density functional theory (DFT); Nanofiltration (NF)

  • Climate-shaped vegetation dominated the spatial pattern of the Bowen ratio over terrestrial ecosystems in China

    作者：Sun, Mingyu;Han, Lang;Yao, Yuan;Wang, Jilong;Yu, Guirui;Chen, Zhi;Hao, Tianxiang;Yang, Meng;Zhang, Weikang;Liu, Zhaogang;Ma, Lexin;Dou, Xiaojun;Luo, Wenxing;Lin, Yong;He, Yongtao;Shi, Peili;Wang, Huimin;Zhang, Yangjian;Zhao, Fenghua;Zhu, Zhilin;Yu, Guirui;Chen, Zhi;Liu, Zhaogang;Ma, Lexin;Dou, Xiaojun;Luo, Wenxing;Lin, Yong;He, Yongtao;Shi, Peili;Zhang, Yangjian;Chen, Zhi;Zhu, Xianjin;Han, Lang;Chen, Shiping;Deng, Zhengmiao;Duh, Hu;Wei, Wenxue;Dong, Gang;Gao, Yanhong;Ji, Xibin;Li, Yuqiang;Tan, Junlei;Gu, Fengxue;Hao, Xiangxiang;Hao, Yanbin;Hen, Qihua;He, Jinsheng;He, Jinsheng;He, Jinsheng;He, Jinsheng;Chen, Shiping;Jiang, Zhengde;Wu, Jiabing;Zhu, Jiaojun;Li, Xinhu;Li, Yingnian;Zhao, Liang;Li, Yuzhe;Zhang, Fawei;Liu, Shaomin;Luo, Weijun;Mo, Xingguo;Zhang, Yiping;Sha, Liqing;Song, Qinghai;Zhang, Yiping;Sun, Dan;Yan, Junhua;Tan, Junlei;Tang, Yakun;Wang, Fei;Wang, Jianlin;Wu, Zhixiang;Xin, Xiaoping;Xin, Xiaoping;Zhang, Yucui;Zhou, Li;Zhang, Weikang;Chen, Zhi

    关键词：Bowen ratio; Heat flux; Sensible heat; Eddy covariance; Spatial patterns; Energy partitioning

  • Cloning and Characterization of Chitin Deacetylase from Euphausia superba

    作者：Wang, Xutong;Tan, Jiahao;Zou, Huaying;Wang, Xutong;Tan, Jiahao;Zou, Huaying;Wang, Fang;Xu, Jiakun;Wang, Fang;Xu, Jiakun

    关键词：chitin deacetylase; Euphausia superba; heterologous expression; enzymatic properties; molecular simulation

  • Enhanced fucoxanthin and biomass accumulation in Phaeodactylum tricornutum by combined effect of two plant hormones

    作者：Lin, Jiaxue;Zhao, Shaogeng;Huang, Beichen;Li, Shengjie;Ma, Yan;Ye, Jingrun;Wang, Zhipeng;Yin, Jianhua;Wang, Haiying;Ji, Xiaofeng;Xu, Jiakun

    关键词：Fucoxanthin; Eicosapentaenoic acid; Phaeodactylum tricornutum; Combined effect; Plant hormones

  • Two hypercrosslinked microporous carbazole-based polymers for electrochemical recognition of penicillin

    作者：Tian, Guanghui;Guo, Feng;Fan, Chuanbin;Zong, Ziao;Wang, Junli;Xu, Jiakun

    关键词：Carbazole; Porous polymer; Aptasensor; Penicillin; Electrochemical impedance spectroscopy

  • The enormous repetitive Antarctic krill genome reveals environmental adaptations and population insights

    作者：Shao, Changwei;Liu, Kaiqiang;Li, Shuo;Wang, Qian;Wang, Hong-Yan;Feng, Bo;Wang, Rui;Hao, Xiancai;Xu, Jiakun;Yue, Bowen;Sun, Yanxu;Liu, Yuyan;Liu, Shufang;Zhao, Xianyong;Shao, Changwei;Liu, Kaiqiang;Li, Shuo;Wang, Qian;Wang, Hong-Yan;Feng, Bo;Wang, Rui;Hao, Xiancai;Yue, Bowen;Sun, Yanxu;Liu, Yuyan;Liu, Shufang;Sun, Shuai;Wang, Jiahao;Liu, Qun;Liu, Yalin;Liu, Shanshan;Sun, Tao;Zhang, Yaolei;Pan, Shanshan;Xu, Mengyang;Xu, Xun;Wang, Jun;Fan, Guangyi;Sun, Shuai;Liu, Xin;Wang, Yue;Huang, Guodong;Ma, Kailong;Zhang, Yaolei;Wang, Yang;Zhang, He;Xu, Mengyang;Zhang, Guojie;Yang, Huanming;Wang, Jun;Fan, Guangyi;Sun, Shuai;Liu, Qun;Deagle, Bruce E.;Deagle, Bruce E.;Kawaguchi, So;Seim, Inge;Biscontin, Alberto;Pitta, Cristiano De;Sales, Gabrielle;Liu, Xin;Liu, Xin;Liu, Xin;Jarman, Simon;Wang, Yue;Wang, Yue;Hu, Jiang;Ma, Kailong;Ying, Yiping;Wang, Xinliang;Zhao, Yunxia;Xu, Jiakun;Zhu, Jiancheng;Zhao, Xianyong;Jia, Xiaodong;Ruan, Jue;Fan, Guangyi;Zhang, Guojie;Yang, Huanming;Meyer, Bettina;Meyer, Bettina;Meyer, Bettina;Fan, Guangyi

    关键词：

  • Construction of Biocatalysts Using the P450 Scaffold for the Synthesis of Indigo from Indole

    作者：Li, Yanqing;Li, Yanqing;Wang, Fang;Wang, Jinghan;Xu, Jiakun;Lin, Yingwu;Wang, Jinghan;Shoji, Osami

    关键词：cytochromes P450BM3; dual-functional small molecule co-catalysis; hydroxylated indole; indigo; directed evolution

微信小程序