Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole

文献类型： 外文期刊

第一作者： Zhang, Weikang

作者： Zhang, Weikang;Xu, Jiakun;Zhang, Weikang;Meng, Xiangmin;Lin, Yingwu;Wang, Qiaoning;Chen, Lingxin

作者机构：

关键词： Sulfonamides; Myoglobin; Biocatalysis; Biodegradation; Directed evolution

期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）

ISSN： 0304-3894

年卷期： 2024 年 465 卷

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收录情况： SCI

摘要： Sulfonamide antibiotics, a family of broad-spectrum antibiotic drugs, are increasingly used in aquaculture and are frequently detected in aquatic environments. This poses a potential threat to organisms and may cause the evolution of antimicrobial resistance. Therefore, it is important to develop an environmentally friendly and efficient biocatalyst to degrade sulfonamides (SAs) such as sulfadiazine (SD) and sulfathiazole (ST). Here, we realized the direct and efficient degradation of SD and ST using a hydrogen peroxide-dependent artificial catalytic system based on myoglobin (Mb). The arrangements of amino acids at positions 29, 43, 64, and 68 were found to influence catalytic activity. An L29H/H64D/V68I myoglobin mutant showed the best catalytic efficiency (i.e., kcat/Km = 720.42 M-1 s-1) against SD. Next, mutant H64D/V68I showed the best degradation rate against SD (i.e., 91.45 +/- 0.16%). Moreover, L29H/H64D/V68I Mb was found to efficiently catalyze ST oxidation

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