tRNA selectivity during ribosome- associated quality control regulates the critical sterility- inducing temperature in two- line hybrid rice

文献类型： 外文期刊

第一作者： Zhou, Can

作者： Zhou, Can;Liu, Chunyan;Yan, Bin;Sun, Jing;Li, Shengdong;Li, Ji;Wang, Jia;Huang, Xiahe;Yang, Shuying;Song, Xianwei;Wang, Yingchun;Qian, Wenfeng;Cao, Xiaofeng;Zhou, Can;Wang, Yingchun;Qian, Wenfeng;Cao, Xiaofeng;Sun, Jing;Yan, Wei;Fu, Chenjian;Qin, Peng;Fu, Xingxue;Zhao, Xinghui;Wu, Yaxian;Yang, Yuanzhu;Yang, Yuanzhu

作者机构：

关键词： critical sterility-inducing temperature (CSIT); ribosome- associated quality control (RQC); C-terminal alanine and threonine modification (CATylation); OsRqc2; tRNA

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：9.1； 五年影响因子：10.6 ）

ISSN： 0027-8424

年卷期： 2025 年 122 卷 6 期

页码：

收录情况： SCI

摘要： The two- line hybrid rice system, a cutting- edge hybrid rice breeding technology, has greatly boosted global food security. In thermo- sensitive genic male sterile (TGMS) lines, the critical sterility- inducing temperature (CSIT; the temperature at which TGMS lines change from male fertile to complete male sterile) acts as a key threshold. We recently uncovered that thermo- sensitive genic male sterility 5 (tms5), a sterile locus presenting in over 95% of TGMS lines, leads to the overaccumulation of 2 ',3 '- cyclic phosphate (cP)-OCCA-tRNAs and a deficiency of mature tRNAs, which underlies the molecular mechanism of tms5- mediated TGMS. However, there are a few reports on the regulatory mechanism controlling CSIT. Here, we identified a suppressor of tms5, an amino acid substitution (T552I) in the rice Rqc2 (ribosome- associated quality control 2), increases the CSIT in tms5 lines through its C- terminal alanine and threonine modification (CATylation) activity.This substitution alters tRNA selectivity, leading to the recruitment of different tRNAs to the A- site of ribosome and CATylation rate by OsRqc2 during ribosome- associated quality control (RQC), a process that rescues stalled ribosomes and degrades abnormal nascent chains during translational elongation. Further, the mutation restores the levels of mature tRNA-Ser/Ile to increase the CSIT of tms5 lines. Our findings reveal the origin of overaccumulated cP-OCCA-tRNAs in tms5 lines, further deepening our understanding of the regulatory network in governing CSIT of TGMS lines containing tms5.

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