Immune modulation of goat monocytes by Fasciola gigantica Legumain-1 protein (Fg-LGMN-1)

文献类型： 外文期刊

第一作者： Ehsan, Muhammad

作者： Ehsan, Muhammad;Hu, Rui-Si;Wang, Meng;Hou, Jun-Ling;Ehsan, Muhammad;Rashid, Muhammad;Malik, Muhammad Irfan

作者机构：

关键词： Fasciola gigantica; Legumains; Monocytes; Host -parasite interactions; Immune -regulation

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.1； 五年影响因子：2.1 ）

ISSN： 0014-4894

年卷期： 2024 年 256 卷

页码：

收录情况： SCI

摘要： Legumains belonging to C_13 peptidase family of proteins, and are ubiquitously disseminated among all vertebrate and invertebrate organisms, and have been implicated in innumerable biological and cellular functionality. Herein, we characterized and evaluated immunoregulatory characteristics of Legumain-1 from Fasciola gigantica (Fg-LGMN-1) during its interaction with host immune cells. The isopropyl-ss-d-thiogalactopyranoside (IPTG) stimulated RFg-LGMN-1 protein was positively detected by rat serum containing anti-RFg-LGMN-1 polyclonal antibodies. Furthermore, the uptake of RFg-LGMN-1 by goat monocytes was successfully confirmed using Immunofluorescence Assay (IFA). The immunohistochemical analysis revealed the native localization of LGMN-1 protein on the periphery and internal structures such as suckers, pharynx, and genital pore of the adult parasite, thereby validating its presence in excretory-secretory (ES) products of F. gigantica. The RFg-LGMN-1 co-incubated with concanavalin-A (Con-A) stimulated the increase of interleukin 2 (IL-2), IL-10, and IL-17 in monocytes derived from peripheral blood mononuclear cells (PBMCs) in the concentration-dependent manner. However, the IL-4 cytokine in response to the RFg-LGMN-1 protein declined. These results illuminated the role of LGMN-1 during the parasite-host interface. Our findings elaborated additional evidence that Legumain protein play a role in the manipulating host immune responses during parasite infections. However, further evaluation of RFgLGMN-1 protein in context of its immunomodulatory roles should be conducted to enhance our understandings of the mechanisms employed by F. gigantica to evade host immune responses.

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