Heterotrophic and autotrophic production of L-isoleucine and L-valine by engineered Cupriavidus necator H16

文献类型： 外文期刊

第一作者： Wang, Lei

作者： Wang, Lei;Tu, Tao;Yao, Bin;Zhang, Jie;Wang, Lei;Yao, Junhu;Yao, Bin;Zhang, Jie

作者机构：

关键词： Carbon dioxide; Branched-chain amino acids; Citramalate pathway; Metabolic engineering; Fed-batch fermentation

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2024 年 398 卷

页码：

收录情况： SCI

摘要： Advancement in commodity chemical production from carbon dioxide (CO 2 ) offers a promising path towards sustainable development goal. Cupriavidus necator is an ideal host to convert CO 2 into high -value chemicals, thereby achieving this target. Here, C. necator was engineered for heterotrophic and autotrophic production of Lisoleucine and L-valine. Citramalate synthase was introduced to simplify isoleucine synthesis pathway. Blocking poly-hydroxybutyrate biosynthesis resulted in significant accumulation of isoleucine and valine. Besides, strategies like key enzymes screening and overexpressing, reducing power balancing and feedback inhibition removing were applied in strain modification. Finally, the maximum isoleucine and valine titers of the best isoleucine-producing and valine-producing strains reached 857 and 972 mg/L, respectively, in fed -batch fermentation using glucose as substrate, and 105 and 319 mg/L, respectively, in autotrophic fermentation using CO 2 as substrate. This study provides a feasible solution for developing C. necator as a microbial factory to produce amino acids from CO 2 .

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