Multiple antibodied based immunoaffinity columns preparation for the simultaneous analysis of deoxynivalenol and T-2 toxin in cereals by liquid chromatography tandem mass spectrometry

文献类型： 外文期刊

第一作者： Li, Yanshen

作者： Li, Yanshen;Chen, Anqi;Mao, Xin;Sun, Mingxue;You, Yanli;Yang, Shupeng;Li, Juan;Wu, Yongning;Jiang, Guibin

作者机构：

关键词： Multiple immunoaffinity columns; Cereals; Deoxynivalenol; T-2 Toxin; UHPLC-MS/MS

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2021 年 337 卷

页码：

收录情况： SCI

摘要： Deoxynivalenol (DON) and T-2 toxin are major trichothecenes contaminated in cereals, which might bring harmful effects to humans. In this research, mixed anti-DON and anti-T-2 mAb were used for multiple immunoaffinity columns (mIACs) preparation. Under the optimal conditions, column capacities were tested at 1280 ng/mL for DON and 1160 ng/mL for T-2 toxin. Regeneration investigation showed mIACs capacities were over 510 ng/mL for DON and 440 ng/mL for T-2 toxin in 10 recycle usages. Good performances were obtained when applying mIACs purification coupled UHPLC-MS/MS for spiked samples with limit of detection at 3-13 mu g/kg and mean recoveries at 79.0-97.6%. Applying to estimate the exposure of DON and T-2 toxin in commercial samples, maize samples were 100% DON positive and rice samples were 40% DON positive while T-2 toxin was negative in all tested samples. The proposed method is reliable and suitable for monitoring DON and T-2 toxin in cereal samples.

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