Cloning, expression, and characterization of a novel superoxide dismutase from deep-sea sea cucumber

文献类型： 外文期刊

第一作者： Li, Yanan

作者： Li, Yanan;Chen, Jiawei;Zhang, Haibin;Yan, Lei;Li, Yanan;Kong, Xue

作者机构：

关键词： Purification; Deep-sea enzyme; The Mariana trench

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 163 卷

页码：

收录情况： SCI

摘要： Superoxide dismutases (SODs) are the first line of defense against oxidative damage caused by reactive oxygen species and are associated with stress tolerance. Accordingly, many studies have focused on SODs, especially those from extreme habitats. In this study, we reported a novel Cu,Zn-SOD from the new species Benthodytes marianensis sp. nov (hereafter denoted as Bm-Cu,Zn-SOD) collected from the Mariana Trench. The purified recombinant enzyme with an approx. Mr. of 38 kDa was intracellular and dimeric, and it expressed activity at an optimal temperature of 30 degrees C and optimum pH of 7.4. The Km and Vmax values of Bm-Cu,Zn-SOD were 0.046 +/- 0.004 mM and 1209.151 +/- 24.057 U/mg, respectively. Under the tested conditions, Bm-Cu,Zn-SOD resisted interference from chemicals (beta-mercaptoethanol and ethylenediaminetetraacetic acid), denaturants (urea and guanidine hydrochloride), and high salinity, showing high kinetic stability. Furthermore, Bm-Cu,Zn-SOD could be activated by high hydrostatic pressure. All these properties suggested adaptation to the deep-sea environment and thus indicated its potential future applications. (C) 2020 Elsevier B.V. All rights reserved.

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