Multifunctional Near-Infrared Fluorescent Probes with Different Ring-Structure Trigger Groups for Cell Health Monitoring and In Vivo Esterase Activity Detection

文献类型： 外文期刊

第一作者： Wang, Jiemin

作者： Wang, Jiemin;Yang, Yuexia;Qian, Jing;Cao, Ting;Cao, Yuping;Qin, Wenwu;Wang, Jiemin;Yang, Yuexia;Qian, Jing;Cao, Ting;Cao, Yuping;Qin, Wenwu;Teng, Zhidong;Guo, Huichen;Teng, Zhidong;Guo, Huichen;Zhang, Liang;Liu, Yun

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关键词： ratiometric; near-infrared fluorescent probes; esterase; live and dead cells; mitochondria; zebrafish and mouse imaging

期刊名称：ACS SENSORS （ 影响因子：7.711； 五年影响因子：8.162 ）

ISSN： 2379-3694

年卷期： 2020 年 5 卷 10 期

页码：

收录情况： SCI

摘要： A series of multifunctional ratiometric near-infrared fluorescent probes (CYOH-3, CYOH-4, CYOH-5, and CYOH-6) for esterase detection are designed by gradually changing the deflection of the plane twist in the molecule. These probes are composed of different ring-structure trigger groups (from threemembered ring to six-membered ring) and the same luminescent group CYOH. These probes show maximum absorption at similar to 585 nm and a fluorescence emission peak at similar to 655 +/- 5 nm. In the presence of esterase, the probes were hydrolyzed to expose the fluorophore CYOH (lambda(abs) = 690 nm, lambda(em) = 710 +/- 5 nm), thus exhibiting ratiometric near-infrared fluorescence. The probe CYOH-6 has lower plane deflection angle and better ratiometric (R = I-710 +/- 5nm/I-657 +/- 4nm) fluorescence properties than probes CYOH-3, CYOH-4, and CYOH-5. CYOH-6 (six-membered ring) has been successfully used to target esterase in mitochondria and distinguish Mi between dead cells (esterase inactivation) and live cells. In addition, CYOH-6 has been well used for monitoring of esterase activity in zebrafish and mice, which proves that these probes have good prospects for clinical biomedical applications.

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