Structural characterization and hypoglycemic activity of an intracellular polysaccharide from Sanghuangporus sanghuang mycelia

文献类型： 外文期刊

第一作者： Cheng, Junwen

作者： Cheng, Junwen;Wei, Hailong;Wang, Yanbin;Huang, Xubo;He, Liang;Yang, Saozong;Song, Jiling;Lu, Na;Liu, Yu;Lv, Guoying;Zhang, Zuofa;Ding, Hongmei

作者机构：

关键词： Sanghuangporus sanghuang; Polysaccharide; Hypoglycemic activity; Structure; AFM

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 164 卷

页码：

收录情况： SCI

摘要： A neutral polysaccharide (SSIPS1) was isolated and purified from cultured mycelia of Sanghuangporus sanghuang by DEAE Sepharose Fast Flow and Sephacryl S-100 columns. Basic monosaccharide composition indicated that SSIPS1 was mainly composed of D-glucose. The results of methylation and 2D-NMR analysis suggested that the glycosidic linkages of SSIPS1 were elucidated to consisted of 1,4-linked alpha-D-glucopyranose (Glcp) residues with two branched points at O-6. The two branches were composed of 1,4-linked alpha-D-Glcp terminated with alpha-D-Glcp, 1,4-linked alpha-D-Glcp and 1,4-linked beta-Galp terminated by alpha-D-Glcp. Moreover, its chain conformation was revealed to present a flexible chain conformation in 0.1 NaNO3 with a hydrodynamic radius and radius of gyration of 3.26 and 6.45 nm by multi-angle laser light scattering, with a single chain of 0.559 nm observed by atomic force microscopy. Further, SSIPS1 exhibited a potential inhibitory activity against a-amylase and alpha-glucosidase, and it had hypoglycemic effects on in vitro insulin resistance of HepG2 cells as well. (C) 2020 Elsevier B.V. All rights reserved.

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