Conformational properties and biological activities of alpha-D-mannan from Sanghuangporus sanghuang in liquid culture

文献类型： 外文期刊

第一作者： Cheng, Junwen

作者： Cheng, Junwen;Wang, Yanbin;Hu, Chuanjiu;He, Liang;Wei, Hailong;Yang, Saozong;Song, Jiling;Lu, Na;Liu, Yu;Lv, Guoying;Zhang, Zuofa

作者机构：

关键词： Sanghuangporus sanghuang; Mannan; Conformation

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 164 卷

页码：

收录情况： SCI

摘要： Sanghuang is a well-known edible and medicinal fungus consumed in Asia for centuries. In the present study, a novel exopolysaccharide named as mannan (SSEPS2) was isolated from the liquid culture broth of Sanghuangporus sanghuang by using ethanol precipitation, anion-exchange chromatography, and size-exclusion chromatography, which was exclusively composed of D-mannose with the molecular weight 9.43 x 10(4) g/mol. The structural analysis suggested that SSEPS2 consisted of 1,3-linked and 1,2-linked alpha-D-mannopyranose (Manp), with substitution at O-6 of 1,2-linked alpha-D-Manp by 1,6-linked alpha-D-Manp residues and terminal alpha-D-Manp residues. The exponent of a (0.58) indicated that SSEPS2 existed in a flexible chain conformation, with a coil-like structure in 0.1 M NaNO3 at 25 degrees C, which was further confirmed by the atomic force microscopy images and a three-dimensional molecular model. It exhibited potential antitumor activity against the growth of HepG2 (human hepatoma) and MCF-7 cell lines in vitro. The IC50 values for inhibiting HepG-2 cells and MCF-7 cells proliferation were 365 and 340 mu g/mL, which showed its potential application in the development of anticancer therapeutics. (C) 2020 Elsevier B.V. All rights reserved.

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