Genome-wide identification of accessible chromatin regions in bumblebee by ATAC-seq

文献类型： 外文期刊

第一作者： Zhao, Xiaomeng

作者： Zhao, Xiaomeng;Su, Long;Xu, Weilin;Sun, Cheng;Schaack, Sarah

作者机构：

期刊名称：SCIENTIFIC DATA （ 影响因子：6.444； 五年影响因子：9.051 ）

ISSN：

年卷期： 2020 年 7 卷 1 期

页码：

收录情况： SCI

摘要： Bumblebees (Hymenoptera: Apidae) are important pollinating insects that play pivotal roles in crop production and natural ecosystem services. Although protein-coding genes in bumblebees have been extensively annotated, regulatory sequences of the genome, such as promoters and enhancers, have been poorly annotated. To achieve a comprehensive profile of accessible chromatin regions and provide clues for all possible regulatory elements in the bumblebee genome, we performed ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) on Bombus terrestris samples derived from four developmental stages: egg, larva, pupa, and adult, respectively. The ATAC-seq reads were mapped to the B. terrestris reference genome, and its accessible chromatin regions were identified and characterized using bioinformatic methods. We identified 36,390 chromatin accessible regions in total, including both shared and stage-specific chromatin accessible signals. Our study will provide an important resource, not only for uncovering regulatory elements in the bumblebee genome, but also for expanding our understanding of bumblebee biology throughout development.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序