Insulin/IGF signaling and TORC1 promote vitellogenesis via inducing juvenile hormone biosynthesis in the American cockroach

文献类型： 外文期刊

第一作者： Zhu, Shiming

作者： Zhu, Shiming;Liu, Fangfang;Zeng, Huanchao;Li, Na;Ren, Chonghua;Qin, Yiru;Li, Sheng;Zhu, Shiming;Liu, Fangfang;Zeng, Huanchao;Li, Na;Ren, Chonghua;Qin, Yiru;Li, Sheng;Zhu, Shiming;Zeng, Huanchao;Li, Na;Li, Sheng;Su, Yunlin;Zhou, Shutang;Wang, Guirong;Palli, Subba Reddy;Wang, Jian

作者机构：

关键词： IIS; TORC1; Juvenile hormone; Dsx; Vitellogenesis

期刊名称：DEVELOPMENT （ 影响因子：6.868； 五年影响因子：7.522 ）

ISSN： 0950-1991

年卷期： 2020 年 147 卷 20 期

页码：

收录情况： SCI

摘要： Vitellogenesis, including vitellogenin (Vg) production in the fat body and Vg uptake by maturing oocytes, is of great importance for the successful reproduction of adult females. The endocrinal and nutritional regulation of vitellogenesis differs distinctly in insects. Here, the complex crosstalk between juvenile hormone (JH) and the two nutrient sensors insulin/IGF signaling (IIS) and target of rapamycin complex1 (TORC1), was investigated to elucidate the molecular mechanisms of vitellogenesis regulation in the American cockroach, Periplaneta americana. Our data showed that a block of JH biosynthesis or JH action arrested vitellogenesis, in part by inhibiting the expression of doublesex (Dsx), a key transcription factor gene involved in the sex determination cascade. Depletion of IIS or TORC1 blocked both JH biosynthesis and vitellogenesis. Importantly, the JH analog methoprene, but not bovine insulin (to restore IIS) and amino acids (to restore TORC1 activity), restored vitellogenesis in the neck-ligated (IIS-, TORC1- and JH-deficient) and rapamycin-treated (TORC1- and JH-deficient) cockroaches. Combining classic physiology with modern molecular techniques, we have demonstrated that IIS and TORC1 promote vitellogenesis, mainly via inducing JH biosynthesis in the American cockroach.

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