Characterization of mycotoxins from entomopathogenic fungi (Cordyceps fumosorosea) and their toxic effects to the development of asian citrus psyllid reared on healthy and diseased citrus plants

文献类型： 外文期刊

第一作者： Qasim, Muhammad

作者： Qasim, Muhammad;Ul Islam, Saif;Bamisile, Bamisope Steve;Wang, Liande;Qasim, Muhammad;Islam, Waqar;Noman, Ali;Khan, Khalid Ali;Hafeez, Muhammad;Hussain, Dilbar;Dash, Chandra Kanta;Akutse, Komivi Senyo;Rizwan, Muhammad;Nisar, Muhammad Shahid;Jan, Saad

作者机构：

关键词： Bioactive compounds; Diaphorina citri; Entomopathogenic fungi; Fungal extraction; Liquid chromatography

期刊名称：TOXICON （ 影响因子：3.033； 五年影响因子：2.689 ）

ISSN： 0041-0101

年卷期： 2020 年 188 卷

页码：

收录情况： SCI

摘要： Entomopathogenic fungi (EPF) produce multiple mycotoxins, which play an essential role in improving fungal pathogenesis and virulence. To characterize various mycotoxins from the crude methanol extract of Cordyceps fumosorosea, a major EPF against various insect pests, we performed ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometer (UPLC QTOF MS) technique, and all compounds were identified through molecular mass and formulae. Bassianolide was assessed against the nymphs and adults of Dicuphorina citri reared on healthy and Huanglongbing (HLB)-diseased Citrus spp. Plants under laboratory conditions. Overall, 17 compounds were identified from the fungal extract and categorized into three groups, i.e. (1) alkaloids (Isariotins A C), (2) peptides (Bassianolide, Beauvemlides, Beauvericin A, Isaridins and Destruxin E) and (3) polyketide (Tenuipyrone). The detected beauverolides (B, C, F, I, Ja) from C. fumosorosea were novel mycotoxins, and their detection intensity was the highest in the fungal extract. Furthermore, bassianolide caused more than 70% and 80% mortality of D. citri nymphs and adults after two days of application, respectively. After three days of chemical application, all nymphal and adult populations of D. citri were killed by bassianolide. However, the mortality rates of both populations, nymphs and adults, were higher on HLB-diseased plants as compared to healthy plants.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序