Development of a Monoclonal Antibody Against Porcine CD163 SRCR5 Domain Which Partially Blocks Infection of PRRSV

文献类型： 外文期刊

第一作者： Zhang, Yujiao

作者： Zhang, Yujiao;Zhang, Kuan;Zheng, Hao;Liu, Changlong;Jiang, Yifeng;Li, Liwei;Li, Guoxin;Yu, Lingxue;Zhou, Yanjun;Tong, Wu;Zhao, Kuan;Tong, Guangzhi;Gao, Fei;Zheng, Hao;Zhou, Yanjun;Tong, Wu;Tong, Guangzhi;Gao, Fei;Du, Nannan

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关键词： PRRSV; epitope; SRCR5; CD163; monoclonal antibody

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.412； 五年影响因子：3.588 ）

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年卷期： 2020 年 7 卷

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收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV), which seriously endangers the world pig industry, invades host cells through receptor-mediated endocytosis involving clathrin. CD163 is an essential receptor for PRRSV during its infection of cells. The scavenger receptor cysteine-rich 5 (SRCR5) domain of the CD163 molecule is necessary for PRRSV infection, and interacts with glycoproteins GP2a and GP4 of PRRSV, allowing the virus to infect the host cells. In this study, a monoclonal antibody (mAb) against the SRCR5-6 region of porcine CD163 was developed, and the target epitope of the mAb was determined as (497)TWGTVCDSDF(506), which is directly adjacent to the ligand-binding pocket (LBP) domain (487-495aa) of CD163. Further study indicated that the mAb could partially block PRRSV infection of its target cells, pulmonary alveolar macrophages. The mAb developed in the study may provide a foundation of antiviral therapy for PRRSV.

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