High-throughput metabolomics method based on liquid chromatography-mass spectrometry: Insights into the underlying mechanisms of salinity-alkalinity exposure-induced metabolites changes in Barbus capito

文献类型： 外文期刊

第一作者： Sun, Yan-Chun

作者： Sun, Yan-Chun;Han, Shi-Cheng;Yao, Ming-Zhu;Wang, Yu-Mei;Geng, Long-Wu;Wang, Peng;Liu, Hong-Bai;Yao, Ming-Zhu;Lu, Wei-Hong

作者机构：

关键词： liquid chromatography-mass spectrometry; metabolic biomarkers; metabolites; metabolomics; pathway

期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）

ISSN： 1615-9306

年卷期： 2021 年 44 卷 2 期

页码：

收录情况： SCI

摘要： It is critical to investigate the adaptive development and the physiological mechanism of fish in external stimulation. In this study, the response of Barbus capito to salinity-alkalinity exposure was explored by high-throughput nontargeted and liquid chromatography-mass spectrometry-based metabolomics to investigate metabolic biomarker and pathway changes. Meanwhile, the biochemical indexes of Barbus capito were measured to discover the chronic impairment response to salinity-alkalinity exposures. A total of 29 tissue metabolites were determined to deciphering the endogenous metabolic changes of fishes during the different concentration salinity-alkalinity exposures environment, which were mainly involved in the key metabolism including the phenylalanine, tyrosine, and tryptophan biosynthesis, arachidonic acid metabolism, pyruvate metabolism, citrate cycle, and glycerophospholipid metabolism. Finally, we found the amino acid metabolism as key target was associated with the endogenous metabolites and metabolic pathways of Barbus capito to salinity-alkalinity exposures. In conclusion, metabolomics is a potentially powerful tool to reveal the mechanism information of fish in various exposure environments.

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