OsHDA710-Mediated Histone Deacetylation Regulates Callus Formation of Rice Mature Embryo

文献类型： 外文期刊

第一作者： Zhang, Haidao

作者： Zhang, Haidao;Guo, Fu;Qi, Peipei;Huang, Yizi;Han, Ning;Bian, Hongwu;Xie, Yongyao;Xu, Lei;Xu, Lin;Zhang, Haidao

作者机构：

关键词： Auxin; Callus formation; Histone deacetylase; Mature embryo; OsARF18; OsHDA710; Rice; Trichostatin A

期刊名称：PLANT AND CELL PHYSIOLOGY （ 影响因子：4.927； 五年影响因子：5.516 ）

ISSN： 0032-0781

年卷期： 2020 年 61 卷 9 期

页码：

收录情况： SCI

摘要： Histone deacetylases (HDACs) play important roles in the regulation of eukaryotic gene expression. The role of HDACs in specialized transcriptional regulation and biological processes is poorly understood. In this study, we evaluated the global expression patterns of genes related to epigenetic modifications during callus initiation in rice. We found that the repression of HDAC activity by trichosuttin A (TSA) or by OsHDA710 mutation (hda710) results in impaired callus formation of rice mature embryo and increased global histone H3 acetylation levels. The HDAC inhibition decreased auxin response and cell proliferation in callus formation. Meanwhile, the transcriptional repressors OsARF18 and OsARF22 were upregulated in the callus of hda710. The chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) analysis demonstrated that the callus of hda710 exhibited enhanced histone H3 acetylation levels at the chromatin regions of OsARF18 and OsARF22. Furthermore, we found that OsARF18 and OsARF22 were regulated through OsHDA710 recruitment to their target loci. In addition, overexpression of OsARF18 decreased the transcription of downstream genes PLT1 and PLT2 and inhibited callus formation of the mature embryo. These resets demonstrate that OsHDA710 regulates callus formation by suppressing repressive OsARFs via histone deacetylation during callus formation of rice mature embryo. This indicates that OsHDA710-mediated histone deacetylation is an epigenetic regulation , way for maintaining auxin response during cell dedifferentiation.

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