The gene encoding lycopene epsilon cyclase of celery enhanced lutein and beta-carotene contents and confers increased salt tolerance in Arabidopsis

文献类型： 外文期刊

第一作者： Yin, Lian

作者： Yin, Lian;Liu, Jie-Xia;Tao, Jian-Ping;Duan, Ao-Qi;Ding, Xu;Xu, Zhi-Sheng;Xiong, Ai-Sheng;Xing, Guo-Ming;Li, Sen;Tan, Guo-Fei

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关键词： Celery; AgLCY-epsilon; Lutein; beta-carotene; Salt tolerance

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

ISSN： 0981-9428

年卷期： 2020 年 157 卷

页码：

收录情况： SCI

摘要： Celery (Apium graveolens L.) is a leafy vegetable of Apiaceae, which is greatly popular because of its rich nutrients. Lutein and beta-carotene are two important carotenoids. Lycopene epsilon cyclase (LCY-epsilon) is a key branch point enzyme in the carotenoid biosynthetic pathway. In this study, we cloned the AgLCY-epsilon gene from celery and overexpressed it in Arabidopsis. The results showed that both lutein and beta-carotene accumulation increased significantly in transgenic Arabidopsis hosting AgLCY-epsilon gene, compared with wild type (WT) plants. The transcription levels of AtPSY and AtCRTLSO genes involved in carotenoids biosynthesis also increased in transgenic lines. One-month-old transgenic Arabidopsis seedlings were treated with 200 mM NaCl. The malondialdehyde (MDA) content in transgenic Arabidopsis plants after salt treatment was significantly lower, and the activities of the two antioxidant enzymes, superoxide dismutase (SOD) and peroxidase (POD), were significantly increased than that of WT plants. Overexpression of AgLCY-epsilon gene showed increased lutein and beta-carotene accumulations, and enhanced salt tolerance in transgenic plants.

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