Identification of a novel epitope specific for Gp85 protein of avian leukosis virus subgroup K

文献类型： 外文期刊

第一作者： Chen, Xueyang

作者： Chen, Xueyang;Wang, Houkun;Fang, Xiaowei;Gao, Keli;Fang, Chun;Gu, Yufang;Liang, Xiongyan;Yang, Yuying;Gao, Yulong;Wang, Xiaomei;Huang, Hongsheng

作者机构：

关键词： Avian leukosis virus; Monoclonal antibody; Linear epitope; Gp85

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：2.046； 五年影响因子：2.217 ）

ISSN： 0165-2427

年卷期： 2020 年 230 卷

页码：

收录情况： SCI

摘要： During the past two decades, avian leukosis virus (ALV) caused tremendous economic losses to poultry industry in China. ALV-K as a newly found subgroup in recent years, which made the control and eradication of ALV more difficult as they were originated from the recombination of different subgroups. To date, specific rapid detection methods refer to ALV-K are still missing. Gp85 is the main structural protein of the virus, which mediates the invasion of host cells by the virus and determinates the classification of subgroups. In this study, we prepared a monoclonal antibody (Mab) named Km3 against Gp85 of ALV-K. Immunofluorescence assay showed that Km3 specifically recognized the strains of ALV-K rather than the strains of ALV-A or ALV-J. To explain the subgroups specificity of Km3, the epitope cognized by the Mab was identified by Western blotting using 15 overlapping fragments spanning the Gp85. Finally, the peptide (129)AFGPRSIDTLSDWSRPQ(145) was identified as the minimal linear epitope recognized by Km3. Alignment of Gp85 from different subgroups showed that the epitope was highly conserved among ALV-K strains, which was quite different from that of the strains from ALV-A,-B and-J. In conclusion, the Mab Km3 may serve as a useful reagent for ALV-K detection and diagnosis in the future.

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