Ultrasound-Assisted Interfacial Immobilization of Lipase on Hollow Mesoporous Silica Spheres in a Pickering Emulsion System: A Hyperactive and Sustainable Biocatalyst

文献类型： 外文期刊

第一作者： Sun, Teng

作者： Sun, Teng;Dong, Zhe;Wang, Jing;Huang, Feng-Hong;Zheng, Ming-Ming

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关键词： Pickering emulsion; Ultrasound assisted; Interfacial activation; Lipase immobilization; Sustainable biocatalyst

期刊名称：ACS SUSTAINABLE CHEMISTRY & ENGINEERING （ 影响因子：8.198； 五年影响因子：8.471 ）

ISSN： 2168-0485

年卷期： 2020 年 8 卷 46 期

页码：

收录情况： SCI

摘要： Lipase immobilization processes have the potential to drive large-scale industrial applications through cost savings. Among them, the Pickering emulsion system has recently emerged as an ecofriendly technique for enhanced enzymatic activity. In this study, a novel and facile immobilization of lipase in an ultrasound-assisted Pickering emulsion system was developed. The lipase loading amount and yield was 177.3 mg/g and 41.3%, respectively. In the enzymatic synthesis of four sterol lipoates, the immobilized lipase (UIP-HMSS@CRL) achieved the highest catalytic efficiency (CE) of 6.4 mmol/g.h among the reported methods, which was 9.1-fold higher than that of free lipase and 32.0-fold higher than that of previous methods. UIP-HMSS@CRL performed with much higher V-m, and lower K-m, indicating the excellent catalytic rate and stronger affinity. Moreover, UIP-HMSS@CRL displayed satisfying thermal stability and recyclability, retaining 88.6% of enzyme activity after 10 cycles. The lipase lid was opened at the oil-water interface by both ultrasound and interfacial synergistic activation effect, which provided easier access to an active site and thus achieved excellent catalytic performance. This ultrasound-assisted interface immobilization would open new opportunities for preparation of high-performance and sustainable biocatalysts.

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