Soybean CHX-type ion transport protein GmSALT3 confers leaf Na+ exclusion via a root derived mechanism, and Cl- exclusion via a shoot derived process

文献类型： 外文期刊

第一作者： Qu, Yue

作者： Qu, Yue;Bose, Jayakumar;Henderson, Sam W.;Wege, Stefanie;Gilliham, Matthew;Guan, Rongxia;Qiu, Lijuan

作者机构：

关键词： abiotic stress; CHX; ion transporter; salinity tolerance; shoot ion exclusion

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：7.228； 五年影响因子：7.791 ）

ISSN： 0140-7791

年卷期： 2021 年 44 卷 3 期

页码：

收录情况： SCI

摘要： Soybean (Glycine max) yields are threatened by multiple stresses including soil salinity. GmSALT3 (a cation-proton exchanger protein) confers net shoot exclusion for both Na+ and Cl- and improves salt tolerance of soybean; however, how the ER-localized GmSALT3 achieves this is unknown. Here, GmSALT3's function was investigated in heterologous systems and near isogenic lines that contained the full-length GmSALT3 (NIL-T; salt-tolerant) or a truncated transcript Gmsalt3 (NIL-S; salt-sensitive). GmSALT3 restored growth of K+-uptake-defective Escherichia coli and contributed towards net influx and accumulation of Na+, K+ and Cl- in Xenopus laevis oocytes, while Gmsalt3 was non-functional. Time-course analysis of NILs confirmed shoot Cl- exclusion occurs distinctly from Na+ exclusion. Grafting showed that shoot Na+ exclusion occurs via a root xylem-based mechanism; in contrast, NIL-T plants exhibited significantly greater Cl- content in both the stem xylem and phloem sap compared to NIL-S, indicating that shoot Cl- exclusion likely depends upon novel phloem-based Cl- recirculation. NIL-T shoots grafted on NIL-S roots contained low shoot Cl-, which confirmed that Cl- recirculation is dependent on the presence of GmSALT3 in shoots. Overall, these findings provide new insights on GmSALT3's impact on salinity tolerance and reveal a novel mechanism for shoot Cl- exclusion in plants.

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