Improved dual luciferase reporter (DLR) assay to determine the protein stability

文献类型： 外文期刊

第一作者： Sun, Yinglu

作者： Sun, Yinglu;Zhao, Jinfeng;Patil, Suyash Bhimgonda;Fang, Jingjing;Liu, Jun;Li, Xueyong

作者机构：

关键词： Dual luciferase reporter system; StrigoQuant biosensor; Relative FF/REN ratio; Protein stability; Protein abundance

期刊名称：ANALYTICAL BIOCHEMISTRY （ 影响因子：3.365； 五年影响因子：3.049 ）

ISSN： 0003-2697

年卷期： 2021 年 612 卷

页码：

收录情况： SCI

摘要： We developed a binary vector co-expressing firefly luciferase (FF) and Renilla luciferase (REN) to detect protein stability in response to different stimuli, and verified the functionality of the vector. The StrigoQuant-like reporter expressing FF and REN in one transcript is a sensitive tool for detecting protein abundance in different genotypes. However, we found that significant differences in the relative FF/REN ratio of empty StrigoQuant vector in different genotypes. Therefore, to determine the actual protein abundance, the relative FF/REN ratio of the protein of interest should be normalized to that of the empty vector.

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